在血,血清和全血中65种生物化学分析物的稳定性
Janet Zhou1, Anselmo Fabros2, Sarah Jane Lam2
1Department of Laboratory Medicine & Pathobiology, 233837 University of Toronto , Toronto, ON, Canada.
Clinical chemistry and laboratory medicine
|March 5, 2024
概括
分析前的稳定性对于准确的实验室结果至关重要. 大多数生物化学分析物保持稳定,但延迟离心会影响全血中的葡萄糖,和AST水平.
科学领域:
- 临床化学 临床化学
- 实验室医学 实验室医学
- 生化分析 生化分析
背景情况:
- 分析前样本的稳定性显著影响实验室结果的准确性.
- 在已公布的分析剂稳定性数据中存在变化.
- 需要对分析前稳定性的标准化评估.
研究的目的:
- 评估65种生化分析物的分析前稳定性.
- 为了评估全血,血清和血的稳定性.
- 使用标准化的方法进行比较.
主要方法:
- 从30名健康志愿者的血液中收集到SST,Li-heparin,K2-EDTA或Na-化物/K-oxalate真空箱中的血液.
- 测试了延迟离心 (8小时,24小时在RT/4°C) 和即时离心 (24小时在RT).
- 计算的百分比偏差 (% PD) 与生物变异极限相比.
主要成果:
- 大多数分析品在测试条件下表现出稳定性.
- 延迟离心术在室温下8小时内显著影响全血中的葡萄糖,和AST.
- 在某些预分析条件下,特定的分析品显示不稳定.
结论:
- 提供了对影响生化分析剂稳定性的分析前变量的关键数据.
- 旨在最大限度地减少由于超出了分析剂稳定性的错误实验室结果.
- 通过识别稳定的分析物,尽管处理延迟,可能会减少重复的瘤切除术.
相关概念视频
Composition of Blood Plasma
4.1K
Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins,...
4.1K
Protein Buffers in Blood Plasma and Cells
757
The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
Certain amino acids can exist in a zwitterion state at a...
757
Serum Laboratory Studies, Stool Test, Breath Test
346
Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
346
Blood Studies for Cardiovascular System III: Serum Lipid Profile
163
Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
163


