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CircNUP54通过促进HuR细胞质输出和稳定BIRC3mRNA来促进肝细胞癌的进展

Chenwei Tang^1,2, Hongkai Zhuang^1,2, Wentao Wang^1,2

¹Department of Hepatobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, 510120, China.

Cell death & disease

|March 5, 2024

概括

一种新型的循环RNA,circNUP54,通过通过HuR蛋白稳定BIRC3mRNA,促进肝细胞癌 (HCC) 的进展. 针对这个circNUP54/HuR/BIRC3轴,为HCC提供了一个潜在的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

循环RNAs (circRNAs) 越来越多地被认为是它们在癌症发展中的作用.
肝细胞癌 (HCC) 中circRNAs的特定机制需要进一步研究.

研究的目的:

为了识别和描述参与HCC病变的新型circRNAs.
阐明 circNUP54 在HCC进展中的功能背后的分子机制.

主要方法:

循环RNA测序以识别HCC中差异表达的循环RNA.
功能性实验 (体外和体内) 来评估circNUP54.5的作用.
RNA下拉,RNA免疫沉降和免疫光测试用于研究分子相互作用.
对HuR/BIRC3/NF-κB信号通路的分析.

主要成果:

hsa_circ_0070039 (circNUP54) 在HCC组织中显著上调,与糟糕的结果相关.
Knockdown 的 circNUP54 抑制了 HCC 的进展,而它的过度表达促进了它.
circNUP54与HuR相互作用,促进其细胞质出口并稳定BIRC3mRNA.
circNUP54通过HuR/BIRC3轴激活NF-κB通路,推动HCC的进展.

结论:

circNUP54通过调节HuR/BIRC3/NF-κB信号通路,在HCC中起到瘤性circRNA的作用.
circNUP54代表了HCC治疗的潜在治疗标.

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