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对lncRNA和mRNA表达数据的整合分析确定DOCK4是老年骨质疏松症的潜在生物标志物

Chengai Wu¹, Chao Wang¹, Bin Xiao²

¹Department of Molecular Orthopaedics, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, China.

BMC medical genomics

|March 5, 2024

概括

研究人员确定DOCK4是老年骨质疏松症的潜在生物标志物. 该基因由特定的lncRNAs和miRNAs调节,为骨质疏松症提供了新的诊断可能性.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
生物标志物发现发现

背景情况:

骨质疏松症 (OP) 是老年人群的重大健康问题.
确定可靠的生物标志物对于早期诊断和老年OP的管理至关重要.

研究的目的:

通过对长非编码RNA (lncRNA) 和信使RNA (mRNA) 表达数据的综合分析,识别老年骨质疏松症的潜在生物标志物.
在骨质疏松症的背景下,探索 lncRNA 和它们的基因之间的调节关系.

主要方法:

对来自老年骨质疏松病例和健康对照者的血液样本进行了RNA测序 (RNA-seq).
使用了差异基因表达分析,共同表达网络分析和 cis-trans 调控分析.
用蛋白与蛋白相互作用 (PPI) 网络分析来识别关键基因.

主要成果:

共有897个差异表达的lncRNAs (DELs) 和1366个差异表达的基因 (DEGs) 被确定.
通过IncRNAs调节的69个候选基因被发现,7个基因通过PPI分析进一步选.
通过两个lncRNA转调的DOCK4基因在骨质疏松症样本中显著上调,并通过ROC分析显示出诊断潜力.

结论:

DOCK4是老年骨质疏松症的潜在诊断生物标志物.
DOCK4是由特定的lncRNA和microRNA (miRNA) 调节的.
已识别的lncRNAs和DOCK4显示为骨质疏松症的诊断生物标志物具有前途.

关键词:

在 DOCK4 中使用 DOCK4.诊断诊断诊断的诊断老年人的骨质疏松症在cnRNA中.这是一个小RNARNA.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...