POU-HD TFs 阻碍了几种人类乳头瘤病毒基因组的复制效率
Martin Kala1, Sofiya Babok1, Nika Mikhailava1
1Institute of Technology, University of Tartu, Tartu, Estonia.
Virology journal
|March 5, 2024
概括
宿主细胞POU-HD转录因子调节人类乳头瘤病毒 (HPV) 复制. 过度表达OCT1,OCT6,BRN5A和SKN1A抑制了HPV复制,而OCT1的淘汰会增强它.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 人类乳头瘤病毒 (HPV) 是一种DNA病毒,需要宿主细胞因子进行复制.
- 宿主细胞蛋白质,特别是转录因子 (TFs) 在调节HPV复制中的精确作用尚未完全理解.
- POU-HD TFs已涉及影响病毒复制,但它们在HPV中的特定功能尚不清楚.
研究的目的:
- 研究POU-HDTFs对相关细胞模型中的HPV基因组复制的影响.
- 阐明POU-HD TFs调节HPV复制的机制,包括对病毒基因表达的影响.
主要方法:
- 在U2OS细胞和具有不同POU-HDTF水平 (过度表达和淘汰) 的初级角质细胞中评估HPV复制.
- 利用复制缺陷的HPV18基因组研究促进体活性和TF介导抑制.
- 使用Oct6突变体来区分DNA结合和转录调节作用.
主要成果:
- OCT1,OCT6,BRN5A和SKN1A在HPV宿主细胞中表达,并在过度表达时抑制HPV复制.
- OCT1的淘汰对HPV复制产生了积极的影响.
- 通过调节控制E1和E2表达的早期促进体,OCT1抑制了HPV复制.
- 通过Oct6介导的HPV18复制的抑制仅取决于其DNA结合活性.
结论:
- 包括OCT1和OCT6在内的POU-HDTF在HPV基因组复制中发挥着重要的调节作用.
- OCT1的抑制作用涉及对复制必不可少的病毒促进体的调节.
- Oct6的DNA结合能力对于其在抑制HPV复制中的作用至关重要.
相关概念视频
DNA Helicases
21.3K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
21.3K
Mechanisms of Retrovirus-induced Cancers
5.1K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.1K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Retroviruses
12.3K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.3K
Viral Mutations
32.3K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.3K
Prokaryotic Transcriptional Activators and Repressors
21.0K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.0K


