叶子日呼吸涉及多个碳来源,并取决于以前的黑暗代谢
Cyril Abadie1,2, Julie Lalande1, Corentin Dourmap1
1Institut de recherche en horticulture et semences, INRAe, Université d'Angers, Beaucouzé, France.
Plant, cell & environment
|March 6, 2024
概括
叶子日呼吸 (Rd) 涉及超越标准代谢的多种代谢途径,利用包括储存储量在内的多种碳来源. 这挑战了光合作用模型中对Rd的简化观点.
科学领域:
- 植物生理学 植物生理学
- 代谢生物化学 代谢生物化学
- 光合作用研究研究 光合作用研究
背景情况:
- 日间呼吸 (Rd) 对于光合作用模型至关重要,但其代谢基础尚不清楚.
- 当前的模型通常认为Rd仅仅反映了"普通"的代谢 (糖解和克雷布斯循环).
- 预测Rd与环境因素的变化受到其代谢基础的不完全知识的阻碍.
研究的目的:
- 在照亮的叶子中研究白天呼吸 (Rd) 的代谢复杂性.
- 为了确定非"普通"的代谢途径,在光合作用过程中促进CO2排放.
- 通过同位素追踪,在明暗条件下比较碳利用率.
主要方法:
- 脉冲追逐实验使用光中CO2在光中,然后是黑暗和明亮的追逐周期.
- 非目标的,以同位素辅助的代谢学来分析代谢途径和碳流.
- 对比C标签模式以区分代谢活动.
主要成果:
- 多个并发的代谢途径,包括氧化酸途径和生物合成,有助于Rd.
- 流量重建低估了Rd,表明未测量的CO2-演变过程.
- 酸盐是克雷布斯循环的核心,在黑暗中从光合作用物中合成,并在光线下从储存中重新调动或合成.
结论:
- 叶日呼吸是一个复杂的过程,涉及各种CO2产生反应.
- Rd由各种碳来源提供燃料,包括独立于当前光合作用的储存碳.
- 这项研究提供了直接证据,挑战了日间呼吸的简化模型.
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