在口腔状细胞癌中,Hippo通路蛋白表达的重要性
Yusuke Amano1, Daisuke Matsubara1,2, Atsushi Kihara1
1Department of Integrative Pathology, Jichi Medical University, Shimotsuke, Japan.
Frontiers in medicine
|March 6, 2024
概括
河马的路径 河马的路径
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 河马通路,包括MST1/2,LATS1/2和YAP1,调节细胞增殖和器官大小.
- 河马通路的失调与各种癌症有关.
- 在口腔状细胞癌 (OSCC) 中,Hippo通路蛋白质的作用仍然在很大程度上未被探索.
研究的目的:
- 为了研究口腔上皮质发育不良 (OED),在位癌 (CIS) 和OSCC中主要的Hippo通路蛋白 (MST1/2,LATS1/2,YAP1) 的表达模式.
- 在OSCC中将蛋白质表达与临床病理特征和瘤进展相关联.
主要方法:
- 在OED (n=7),CIS (n=14) 和OSCC (n=109) 组织样本中对MST1,MST2,LATS1,LATS2和YAP1表达的免疫组织化学分析.
- 蛋白质表达与瘤分化,侵袭模式,表皮细胞转化为介质细胞转化 (EMT) 标记物 (E-cadherin,vimentin,laminin 5,Slug) 和蛋白质氨酸甲基转移酶 (PRMTs) 的相关性.
主要成果:
- 在OED,CIS和OSCC中,MST1,LATS1和LATS2的细胞质表达通常很低.
- 在OED,CIS和差异化OSCC中,MST2表达高,但在差异化OSCC中低/丢失.
- YAP1表达与瘤分化有关,在分化良好的OSCC中较低.
- 高MST2和YAP1表达与透性入侵模式相关.
- 高YAP1表达与EMT特征和高PRMT1/5表达有关.
结论:
- MST2在差异化OSCC中表现出明显的表达模式,这表明它在瘤进展中扮演着上下文相关的角色.
- 增高的YAP1表达可能意味着侵略性OSCC与EMT在入侵前线,可能由PRMT调解.
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