来自印度西部的Giardia intestinalis的分子评估:一个前性的观察研究
Naila Mohammad1, Vibhor Tak1, Gopal Krishna Bohra2
1Department of Microbiology, AIIMS, Jodhpur, Rajasthan, India.
Tropical parasitology
|March 6, 2024
概括
在印度患者中,Giardia intestinalis组合B占主导地位,在儿童中观察到显著的衰老和衰竭. 这凸显了这种寄生虫感染的潜在报告不足和动物传播风险.
科学领域:
- 医学寄生虫学 医学寄生虫学
- 分子流行病学分子流行病学
- 公共卫生 公共卫生
背景情况:
- 肠道是一种全球流行的原生动物,会引起胃肠炎.
- 它包括至少八个组合 (基因型).
- 在印度,巨病往往被低估,特别是在无症状个体中.
研究的目的:
- 从印度拉贾斯坦邦的患者中分离出Giardia intestinalis的基因型.
- 建立与已识别的基因型的临床相关性.
主要方法:
- 在第三级护理中心进行前性试点横截面研究 (2019-2021年).
- 在注册患者中显微镜证实了giardiasis.
- 提取DNA,PCR放大以4E1-HP为目标,并对Giardia intestinalis进行基因定型.
- 使用Anthrocal应用对儿科患者进行人体测量测量.
主要成果:
- 在94% (47/50) 的便样本中检测到Giardia intestinalis的B组合.
- 在36% (18/50) 的患者中出现了腹;6人患有并发症.
- 在12岁以下和5岁以下的儿童中,分别观察到显著的衰退 (55.17%) 和衰竭 (44.4%).
结论:
- 组合B是这个印度队列中Giardia intestinalis的主要基因型.
- 这些发现表明潜在的报告不足和动物传播的可能性.
- 临床相关性揭示了受影响儿童的显著生长异常.
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