在大鼠大脑中,尼特尔基系统的反应被条件为内自我刺激
Amit G Choudhary1, Sanjay N Awathale1, Biru B Dudhabhate1
1Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India.
Journal of neurochemistry
|March 6, 2024
概括
涉及神经元氧化合成酶 (nNOS) 的基系统,影响奖励处理和目标导向行为. 抑制nNOS会降低奖励通路中的活性,影响多巴胺水平.
科学领域:
- 神经科学是一个神经科学.
- 神经化学 神经化学
- 行为神经科学 行为神经科学
背景情况:
- 已知基系统在奖励处理上的精神刺激作用中的作用.
- 然而,它的解剖学连接和与中临界多巴胺系统的相互作用需要进一步澄清.
研究的目的:
- 在奖励处理的背景下,研究神经元氧化合成酶 (nNOS) 的解剖学定位和功能意义.
- 阐明和多巴胺系统在调解目标导向行为中的相互作用.
主要方法:
- 免疫组织化学测绘nNOS含有细胞组的图谱,用于进行内自我刺激 (ICSS) 的老鼠.
- 分析相关大脑区域的nNOS mRNA表达.
- 药理上抑制nNOS使用7-nitroindazole (7-NI) 进行脑内静脉内 (icv) 给药.
- 使用微透析测量核贝 (AcbSh) 中的多巴胺 (DA) 和DOPAC水平.
主要成果:
- 增加的nNOS免疫活性在关键的奖励区域如腹膜区域 (VTA) 和核 (AcbSh) 中观察到.
- 在其他大脑区域也观察到nNOS上调,包括核心核 (AcbC),横向下丘脑 (LH) 和周水管灰 (PAG).
- 在ICSS任务中,以剂量为依赖的7-NI的脑内管内给药降低了杆压力活动.
- 服用前7-NI减轻了ICSS大鼠AcbSh中DA和DOPAC流量的增加.
结论:
- 识别特定的基中心,参与调解目标导向行为的感觉,认知和运动方面.
- 证明了nNOS在调节奖励处理和多巴胺释放中的关键作用.
- 突出了与奖励相关的行为中和多巴胺系统之间的功能相互作用.
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