最近在中国发现的肠道病毒C116的鉴定和遗传特征
Zhen-Zhi Han1,2,3, Ji-Chen Li2,3, Jin-Bo Xiao2,3
1Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, China.
Journal of medical virology
|March 6, 2024
概括
肠道病毒C116 (EV-C116) 菌株在健康儿童中被检测到. 这些新型EV-C116菌株表现出重组,并且可以在细胞培养中传播,挑战了以前对其未培养性质的理解.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 肠道病毒C116 (EV-C116) 是一种新兴的肠道病毒C成员,与传染病有关.
- 关于EV-C116的数据有限,尽管在临床样本中偶尔发现了全球性检测.
- 了解EV-C116的多样性和行为对于疾病监测至关重要.
研究的目的:
- 研究EV-C116.6的遗传多样性和特征.
- 探索EV-C116在细胞培养中的传播和细胞病变效应的潜力.
- 了解重组在EV-C多样性中的作用.
主要方法:
- 从看似健康的儿童身上获得的便样本的转录基因组测序.
- 使用最大概率来确定EV-C116血统和基因组的遗传学分析.
- 在各种细胞系 (HEp-2,L20B,VERO,293T,拉布多米索尔科马) 中进行细胞培养传播尝试.
主要成果:
- 两个EV-C阳性的便样本显示出少量的EV-C116菌株.
- 遗传学分析确定了三个EV-C116基因组,研究菌株形成了不同的血统.
- 在EV-C116菌株中观察到重组事件,有助于生物多样性;在没有细胞病变效应的狂宫肌肉瘤细胞中发生传播.
结论:
- EV-C116菌株继续在全球传播,表现出显著的遗传多样性.
- EV-C116在细胞培养中传播的能力,尽管在低位数中没有细胞病变效应,但挑战了其"未培养"的地位.
- 重组在C型肠病毒物种的进化和多样性中起着至关重要的作用.
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