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circ_HMGCS1通过miR-338-5p/IL-7通路调节肝细胞癌的化学抵抗

Siyu Zhang^1,2, Jun Ma¹, Tingdong Yu¹

¹Department of Hepatobiliary and Pancreatic Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, China.

Journal of cellular and molecular medicine

|March 6, 2024

概括

肝细胞癌 (HCC) 化学抵抗与circ_HMGCS1有关,这影响了miR-338-5p/IL-7通路. 抑制这种途径可以克服化学抵抗并改善HCC治疗结果.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

肝细胞癌 (HCC) 对全球健康构成重大挑战,死亡率高.
在HCC患者中,化学抵抗往往导致预后不佳,潜在的机制需要阐明.
循环RNAs (circRNAs) 在HCC化学抵抗中的作用在很大程度上是未知的.

研究的目的:

为了研究circ_0072391 (circ_HMGCS1) 在肝细胞癌中对西斯普拉丁耐药性的作用.
为了阐明涉及circ_HMGCS1,microRNA-338-5p (miR-338-5p) 和HCC化学抵抗中的互白素-7 (IL-7) 的分子机制.
探索向circ_HMGCS1/miR-338-5p/IL-7通路的潜力,以治疗HCC.

主要方法:

定量实时PCR测量cisplatin耐药HCC细胞中的circ_HMGCS1表达.
RNA免疫沉降和双化酶记者测定证实circ_HMGCS1和miR-338-5p之间的相互作用.
西部斑点分析以评估IL-7和下游目标的蛋白质水平.
细胞活力测试用于评估circ_HMGCS1抑制对西斯普拉丁抗性的影响.

主要成果:

Circ_HMGCS1的表达在抗西斯普拉丁的HCC细胞中显著上调.
沉默circ_HMGCS1表达减弱了HCC细胞中的西斯普拉丁耐药性.
Circ_HMGCS1充当了miR-338-5p的微RNA海绵,这反过来调节了IL-7的表达.
鉴定出circ_HMGCS1/miR-338-5p/IL-7通路是HCC化学抵抗的关键调节者.

结论:

Circ_HMGCS1通过miR-338-5p/IL-7通路促进肝细胞癌的化学抵抗.
抑制circ_HMGCS1,miR-338-5p或IL-7可能是克服HCC化学抵抗的治疗策略.
作为一种免疫疗法点,IL-7在HCC治疗中增强免疫功能.

关键词:

circ_HMGCS1/miR-338-5p/IL-7 在线观看西斯普拉丁的化学抵抗性肝细胞癌是一种肝细胞癌.免疫疗法免疫疗法

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