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Updated: Jul 1, 2025

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

用LC-MS/MS进行样本校准和绝对量化的一种单一同位素

Soroush Torkamannejad¹, Ge Chang¹, Fabusuyi A Aroge²

¹Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A1S6, Canada.

Journal of proteome research

|March 6, 2024

概括

这项研究引入了一种简化质谱法 (MS) 方法,使用内源性来准确量化. 沉重匹配光 (HML) 技术通过简化复杂生物样本的校准来增强生物标记物的发现.

科学领域:

发现生物标志物的发现.
分析化学是一种分析化学.
质谱法 (MS) 是一种质谱法.

背景情况:

基于向质谱 (MS) 的绝对定量分析对于生物标志物发现至关重要.
目前的方法依赖于复杂的校准,使用替代品和内部标准的多个同位素.
精确的样本校准是通过MS的精确质量测量能力实现的.

研究的目的:

为了简化基于质谱 (MS) 的绝对定量分析的样本校准.
引入一种新的方法",重匹配光" (HML),用于直接量化内源分析物.
评估HML方法对蛋白质,和小分子的适用性.

主要方法:

利用内源光作为简化校准的内部标准.
开发了一个数学推理",重相匹配光" (HML),用于直接量化.
应用HML方法来量化心脏组织消化和恢复效率,使用尖端蛋白质.

主要成果:

在真实矩阵中,HML方法提供了基本的测试性能参数 (LLOQ,校准曲线截图).
量化只需要分析物的单一同位素,简化了这个过程.
证明了测试在量化蛋白质消化和恢复效率方面的稳定性.

结论:

HML方法提供了一种简化和强大的方法,用于使用向质谱 (MS) 进行绝对定量分析.
这种技术是多用途的,适用于广泛的分析物,包括蛋白质,和小分子.
该方法通过简化校准和提高复杂生物矩阵的测试性能来增强生物标记物的发现.

关键词:

绝对的蛋白质量定量.消化和恢复效率的消化和恢复效率.在样本内进行校准.膜蛋白质是一种膜蛋白质.平行反应监测 (PRM) 是指对反应进行平行监测.稳定同位素稀释的稳定同位素稀释替代分析物方法替代分析物方法替代矩阵方法的替代矩阵方法.有针对性的质谱测量.

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