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Updated: Jul 1, 2025

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Published on: April 27, 2018

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光蛋白CGP与in situ生物合成的非正规氨基酸的定向进化

Yanhong Yang1, Jing Zhang1, Jian Yang1

  • 1School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.

Applied and environmental microbiology
|March 6, 2024
PubMed
概括

生物合成非正规氨基酸 (ncAAs) 并在现场用于蛋白质工程,为化学合成提供了具有成本效益的替代方案. 这种方法增强了蛋白质的功能和稳定性,为生物工程中的更广泛应用铺平了道路.

关键词:
光蛋白是一种光蛋白质.遗传密码扩张 扩张代谢工程是代谢工程.非正规的氨基酸.蛋白质工程工程 蛋白质工程

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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