通过调节miR-129-5p/TNFSF10轴,LncRNA XIST促进了膀癌的进展
Yu-Lin Kong1, Hui-Dan Wang1, Meng Gao1
1Department of Epidemiology, School of Public Health, Mudanjiang Medical University, 3 Tong Xiang Street, Mudanjiang, 157011, Heilongjiang, China.
Discover oncology
|March 6, 2024
概括
长非编码RNA XIST在膀癌 (BC) 中升高,并驱动其进展. 用miR-129-5p抑制XIST及其ceRNA机制来调节TNFSF10显示了早期BC诊断的潜力.
科学领域:
- 分子瘤学分子瘤学
- 癌症基因组学 癌症基因组学
- 生物标志物发现发现
背景情况:
- 研究长非编码RNA XIST在膀癌 (BC) 中的作用对于了解疾病机制至关重要.
- 阐明涉及XIST,microRNA-129-5p和TNFSF10的ceRNA调节网络,可以深入了解BC病原性.
- 评估XIST及其相关分子用于早期BC检测的临床实用性是一个重要的临床需求.
研究的目的:
- 研究IncRNA XIST在膀癌中的差异表达和生物功能.
- 阐明竞争的内源RNA (ceRNA) 调控机制,其中涉及lncRNA XIST,miR-129-5p和TNFSF10.
- 评估IncRNA XIST,miR-129-5p和TNFSF10在早期膀癌检测中的诊断价值.
主要方法:
- 使用定量实时PCR (qRT-PCR) 来确定lncRNA XIST,miR-129-5p和TNFSF10的表达水平.
- 用CCK8,伤口愈合和Transwell测试来评估细胞增殖,迁移和入侵.
- 生物信息学分析和双路西法酶记者测定被用来确认ceRNA网络内的相互作用.
主要成果:
- 在膀癌细胞系和组织中,LncRNA XIST和TNFSF10显著上调,而miR-129-5p在膀癌细胞系和组织中显著下调.
- lncRNA XIST的耗尽抑制了膀癌细胞的增殖,迁移和入侵,表明其致癌作用.
- LncRNA XIST作为miR-129-5p的海绵,从而调节TNFSF10的表达. 对XIST,miR-129-5p和TNFSF10的综合分析表明,膀癌的诊断准确性高 (AUC=0.900).
结论:
- LncRNA XIST是膀癌的致癌驱动因素,其抑制可以阻碍瘤的进展.
- 在lncRNA XIST/miR-129-5p/TNFSF10轴代表一个新的ceRNA调节途径在膀癌.
- 作为早期诊断膀癌的生物标志物,LncRNA XIST,miR-129-5p和TNFSF10具有显著的潜力.
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