从酸面粉和不同传统乳制品中分离出的LAB的益生菌表征,使用生物化学,分子和计算方法
Yousef Nami1, Bahman Panahi2, Hossein Mohammadzadeh Jalaly2
1Department of Food Biotechnology, Branch for Northwest and West Region, Agricultural Research, Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran, 29 Bahman Boulevard, P. O. Box 5156915598, Tabriz, Iran. yousefnami2010@gmail.com.
Probiotics and antimicrobial proteins
|March 6, 2024
概括
伊朗土著酸面粉和乳制品样本产生了有前途的乳酸细菌 (LAB),具有显著的益生菌特征. 这些LAB菌株由于其生存,粘附和胆固醇同化能力,显示出功能性食品的潜力.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 益生菌 益生菌 益生菌
背景情况:
- 乳酸细菌 (LAB) 在发酵食品中至关重要.
- 来自伊朗来源的本土LAB的益生菌潜力尚未得到充分探索.
- 功能性食品需要强大的微生物候选者,具有已证明的健康益处.
研究的目的:
- 从伊朗酸面粉和乳制品中分离和识别LAB.
- 为了评估这些LAB分离物的体外益生菌特性.
- 评估它们作为候选功能性食品应用的适用性.
主要方法:
- 从当地样本中选了560个潜在的LAB分离物.
- 在实验室评估模拟胃肠道条件下的生存情况.
- 评估血液溶解活性,聚合特性,疏水性,胆固醇同化,病原体基因缺失,细胞粘附,生物膜形成和外聚糖的产生.
主要成果:
- 87个LAB分离体显示出高生存率和没有血液溶解活性.
- 选择的分离体显示出显著的自身聚合,协同聚合和细胞表面的疏水性.
- 19个分离物吸收了30%以上的胆固醇;40个分离物是非致病的,并且很好地附着在肠道细胞中;大多数分离物产生了外聚糖.
结论:
- 来自伊朗酸面粉和乳制品的本土LAB具有宝贵的益生菌特性.
- 这些分离物体在体外表现出有希望的功能性质,包括病原体抑制和胆固醇管理.
- 已识别的LAB菌株是开发用于功能性食品的益生菌的强有力的候选人.
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