通过对差异表达的lincRNA和编码基因的权重基因共同表达网络分析,揭示长跨基因非编码RNA (lincRNA) 和与HPV16相关的宫癌病原体之间的功能关联
Abarna Sinha1, Sahana Ghosh1, Abhisikta Ghosh1
1National Institute of Biomedical Genomics, Kalyani, West Bengal, India.
Carcinogenesis
|March 6, 2024
概括
长跨基因非编码RNAs (lincRNAs) 与HPV16阳性宫癌有关. 特定的lincRNA模块与患者的结果和疾病途径相关,其中一个lincRNA影响生存率.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 长跨基因非编码RNAs (lincRNAs) 是基因表达的关键调节者,并与疾病的发病有关.
- 人类乳头瘤病毒16型 (HPV16) 是导致子宫癌 (CaCx) 的首要原因.
研究的目的:
- 调查lincRNAs与HPV16阳性宫癌的发病之间的关联.
- 识别lincRNA编码基因共同表达网络及其与临床特征的相关性.
主要方法:
- 权重基因共同表达网络分析 (WGCNA) 用于分析lincRNA和编码基因表达数据.
- 不同表达分析比较HPV16阳性CaCx患者与HPV阴性对照.
- 相关性分析将基因模块与患者特征联系起来,并在TCGA-CESC数据集中验证了这些发现.
主要成果:
- 确定了五个差异表达的lincRNA (DElincG) 模块,它们与差异表达的编码基因 (DEcG) 共同表达和相关.
- 四个模块与HPV16状态,淋巴结参与和整体存活率 (OS) 有显著的相关性.
- 林克RNA CTD-2619J13.13的下调与糟糕的生存系统有关,并与角质化损失和角质细胞分化通路有关.
结论:
- lincRNAs在HPV16阳性宫癌的发病过程中发挥着重要作用.
- 特定的lincRNA模块和枢纽基因,如CTD-2619J13.13,是潜在的预后生物标志物.
- 这些发现对于对抗高死亡率癌症,特别是在发展中国家,具有翻译意义.
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