Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.0K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.0K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

668
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
668
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

1.4K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
1.4K
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

2.3K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
2.3K
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

1.5K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
1.5K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

707
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
707

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

GFP chromophore photophysics: ultrafast dynamics and hot ground state cooling in the neutral form.

Chemical science·2026
Same author

Rhodanine to Oxorhodanine Switch Switches Switching Mechanism in a Monomethine Photoswitch.

The journal of physical chemistry letters·2026
Same author

Ultrafast Excited-State Dynamics of Dithienyltetrazine-Based Donor-Acceptor Copolymers.

The journal of physical chemistry. B·2026
Same author

Anti-Kasha emission in DCM-IFC: computational evaluation of the type III separated wavefunction hypothesis.

Physical chemistry chemical physics : PCCP·2026
Same author

Conformational switching modulates excited-state pathways in a cofacial perylene dimer.

Chemical science·2026
Same author

Ultrafast Kerr Spectroscopy Reveals Bulk-Like Solvent Dynamics in Concentrated LiTFSI-Acetonitrile Electrolytes.

ChemPlusChem·2026
Same journal

Accurate Density Functional Theory Forces for Charged Noncovalent Complexes.

The journal of physical chemistry letters·2026
Same journal

Dopant-Centered versus Intersite Synergistic Mechanisms in H<sub>2</sub> Dissociation on Single-Atom Alloys.

The journal of physical chemistry letters·2026
Same journal

Post-Translational Modification as an Allosteric Switch in Hsp90: How Dual Phosphorylation Locks Chaperone Complexes into Hyperstabilized States.

The journal of physical chemistry letters·2026
Same journal

LHCSR1 Functions as a Dimmer Switch for Light Harvesting.

The journal of physical chemistry letters·2026
Same journal

Sparse Linear Surrogates Match Neural Network Potentials on the SPICE Biomolecular Benchmark with Three Orders of Magnitude Smaller Training Sets.

The journal of physical chemistry letters·2026
Same journal

Solid-State NMR Quantification of Brønsted-Lewis Acid Site Cooperativity in Zeolites for Glucose Conversion.

The journal of physical chemistry letters·2026
查看所有相关文章

相关实验视频

Updated: Jul 1, 2025

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.5K

二维电子光谱学解决了相对激发状态的相对位移.

Giovanni Bressan1, Dale Green1, Garth A Jones1

  • 1School of Chemistry, Norwich Research Park, University of East Anglia, Norwich NR4 7TJ, United Kingdom.

The journal of physical chemistry letters
|March 6, 2024
PubMed
概括
此摘要是机器生成的。

超快的二维电子光谱揭示了分子潜在能量表面之间的振动模式移位. 这项技术量化了核波束动态,以获得增强的光谱和光化学洞察力.

更多相关视频

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

17.6K
Excitonic Hamiltonians for Calculating Optical Absorption Spectra and Optoelectronic Properties of Molecular Aggregates and Solids
08:04

Excitonic Hamiltonians for Calculating Optical Absorption Spectra and Optoelectronic Properties of Molecular Aggregates and Solids

Published on: May 27, 2020

8.4K

相关实验视频

Last Updated: Jul 1, 2025

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.5K
Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

17.6K
Excitonic Hamiltonians for Calculating Optical Absorption Spectra and Optoelectronic Properties of Molecular Aggregates and Solids
08:04

Excitonic Hamiltonians for Calculating Optical Absorption Spectra and Optoelectronic Properties of Molecular Aggregates and Solids

Published on: May 27, 2020

8.4K

科学领域:

  • 化学物理 化学物理
  • 分子光谱学 分子光谱学
  • 摄影化学的使用.

背景情况:

  • 了解潜在能量表面 (PES) 之间的相对位移对于光谱学和光化学至关重要.
  • 振动连贯编码有关这些位移的信息.
  • 超快速光谱技术对于探测分子动力学至关重要.

研究的目的:

  • 在探头半宽带 (HB2DES) 几何学中应用超快的二维电子光谱法,以研究紫色的基态和兴奋状态潜在景观.
  • 在多个 PES 中提取定量多维,振动坐标信息.

主要方法:

  • 使用超快的二维电子光谱在一个探头半宽带 (HB2DES) 几何.
  • 分析2D连贯图,以识别和描述振动模式.
  • 模拟实验数据,使用三级位移波器模型,使用运动相匹配方法 (HEOM-PMA) 的等级方程.

主要成果:

  • 2D连贯性地图显示了585厘米Raman-active模式在基态区域和338厘米Raman-active模式在兴奋状态吸收中增强的独特本地化.
  • HEOM-PMA建模表明,与338厘米-1坐标相比,在585厘米-1坐标沿线的潜在能量表面位移大于S1 ←S0.
  • 沿着两个振动坐标观察到类似的S2 ← S1位移.

结论:

  • HB2DES是一个强大的工具,用于探测核波袋动态.
  • 这种方法使多维振动坐标信息在多个潜在能量表面的定量提取成为可能.
  • 这项研究为电子状态和核运动之间的合提供了关键的见解.