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工程站点228的Streptomyces coelicolor Laccase用于优化催化活动

Luyao Bian¹, Silu Zhang¹, Tingting Chang¹

¹Laboratory of Food Industrial Enzyme Technology, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, P. R. China.

Journal of agricultural and food chemistry

|March 6, 2024

概括

研究人员设计了一种小乳糖酶 (SLAC) 酶,以降解有害污染物马六甲绿 (MG). 突变氨基酸228显著增强了酶活性和MG脱色,提供了生物修复的潜力.

科学领域:

生物化学生物化学
环境科学环境科学
酶学是一种酶学.

背景情况:

马六合绿 (MG) 是一种有毒的染料,具有重大环境和健康风险.
乳酸酶是具有降解像MG这样的污染物潜在的酶.
调节乳糖酶活性对于有效的生物修复策略至关重要.

研究的目的:

为了研究来自*Streptomyces coelicolor*的小乳糖酶 (SLAC) 的催化活性调节.
专注于氨基酸位置228在SLAC的功能和MG降解中的作用.
探索工程SLAC的潜力,以酶为马拉绿的生物修复.

主要方法:

在SLAC的氨基酸位置228使用和突变发生.
对野生类型 (WT) 和突变酶进行了酶动力学 (Km,kcat) 和结构分析.
进行了马六合绿色脱色试验,以评估生物修复效率.

主要成果:

产生了5种突变,其特异性活性增加了50%.
与WT相比,突变E228F显示了8.8%的Km,而E228T显示了133%的kcat增加.
突变物,特别是E228G,表现出增强的马拉绿色色变色.

结论:

氨基酸位置228是SLAC功能调节的关键控制开关.
工程 SLAC 变种表现出改善的催化性能和增强的 MG 降解.
这项研究为SLAC工程和酶生物修复应用提供了有希望的见解.

关键词:

马六合岩绿色降解退化这种菌种名为Streptomyces laccase.催化活动的催化活动.突变是一种突变.

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