基于基托桑纳米颗粒的口服沙门氏菌肠杆菌亚单位疫苗在 brojlers 中引起了对沙门氏菌 typhimurium 的交叉保护
Sara Dolatyabi1, Sankar Renu1, Jennifer Schrock1
1Center for Food Animal Health, Department of Animal Sciences; The Ohio State University, Wooster, OH 44691, USA.
Poultry science
|March 6, 2024
概括
一种新型口服疫苗使用Salmonella enteritidis (SE) 外膜蛋白和纳米颗粒中的鞭毛素显示出对 brojlers中的Salmonella typhimurium (ST) 的交叉保护,为现有疫苗提供了潜在的替代方案.
科学领域:
- 兽医免疫学 兽医免疫学
- 禽类的健康状况
- 疫苗的研发工作正在进行中.
背景情况:
- 非伤寒型沙门氏菌感染在家禽业中带来了重大的经济和健康挑战.
- 开发有效的口服疫苗以广泛保护沙门氏菌血清型仍然是一个挑战.
- 现有的沙门氏菌疫苗通常需要在诱导强大的粘膜免疫力方面进行改进.
研究的目的:
- 为了评估口服Salmonella enteritidis (SE) 抗原基纳米颗粒疫苗对Salmonella typhimurium (ST) 的交叉保护疗效,在 brojlers.
- 将纳米粒子疫苗引起的免疫反应与商业性ST疫苗进行比较.
- 评估用新型口服SE疫苗接种的 brojlers 中ST殖民性的减少.
主要方法:
- 沙门氏菌肠炎 (SE) 免疫外膜蛋白 (OMP) 和鞭毛蛋白 (FLA) 被困在曼诺基托纳米颗粒 (OMP-FLA-mCS NPs) 中的口服,使用主要增强疗法.
- 在疫苗接种后,在 brojlers 中进行Salmonella typhimurium (ST) 挑战注射.
- 测量特定的分泌IgA和IgY抗体反应.
- 量化ST细菌负载的Cecal含量.
主要成果:
- 与商业Poulvac ST疫苗相比,OMPs-FLA-mCS NPs疫苗诱导的特定抗体反应 (分泌IgA和IgY) 显著更高.
- 新型口服SE疫苗显示,对挑战性ST细菌负载 (0.8 log10 CFU) 的降低,与商用疫苗相比,可以比较.
- OMPs-FLA-mCS-NP疫苗成功地引起了对ST的交叉保护性粘膜免疫反应.
结论:
- 口服的OMPs-FLA-mCS-NP SE疫苗有效地引起了针对肉中的沙门氏菌 typhimurium殖民的交叉保护性粘膜免疫反应.
- 这种以纳米粒子为基础的疫苗显示出潜在的潜力,可以作为一种可行的替代方案,用于目前的禽类活体和死亡的沙门氏菌疫苗.
- 该研究强调了基于纳米颗粒的口服疫苗在家禽行业广泛控制沙门氏菌的潜力.
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