增强基于PLCL的神经导管的神经诱导活动,通过本地上腺体的整合
Xiao Li1, Xiaoyan Mao1, Meihan Tao1
1Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, Shenyang, Liaoning, China.
Biomaterials advances
|March 6, 2024
概括
这项研究开发了一种新的双层神经管道,使用脱细胞化表皮和生物相容聚合物. 这种创新的导管显著提高了老鼠的神经再生,为传统的神经移植提供了一个有希望的替代方案.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 自主神经移植是外围神经修复的标准,但有诸如捐赠者部位的发病率和有限的可用性等限制.
- 基于聚合物的神经管提供了加工优势,但往往由于生物相容性不佳而受到损害.
- 脱细胞化上腺体 (DEP) 保持了生物活性,同时降低了免疫性,为神经修复提供了潜在的生物材料.
研究的目的:
- 创建和评估一种新型的双层神经管道,将脱细胞化上腺体 (DEP) 和多分子乳酸-co-ε-caprolactone (PLCL) 结合起来.
- 评估已开发的双层神经管的物理性质,生物安全性和体内神经再生功效.
主要方法:
- 从哺乳动物的坐骨神经中制备脱细胞化上腺体 (DEP).
- 通过将PLCL膜电旋转到DEP上来制造双层神经管道.
- 在大鼠坐骨神经缺陷模型中评估DEP组件,导管物理性质,生物安全性和功能恢复.
主要成果:
- 双层神经管显示出出色的生物相容性和机械性能.
- 在体内研究表明,与纯PLCL导管相比,神经再生的治疗效果明显优越.
- 证实DEP组件保存良好,并有助于提高性能.
结论:
- 发达的双层神经管道代表了对外围神经修复的有希望的策略.
- 这种方法为自身神经移植提供了一个可行的替代方案,具有更好的临床潜力.
- 对这种生物材料的进一步研究有望促进神经再生疗法的发展.
更多相关视频
08:05Transplantation of Schwann Cells Inside PVDF-TrFE Conduits to Bridge Transected Rat Spinal Cord Stumps to Promote Axon Regeneration Across the Gap
Published on: November 3, 2017
7.0K
10:35Fabrication of the Composite Regenerative Peripheral Nerve Interface C-RPNI in the Adult Rat
Published on: February 25, 2020
8.2K
相关概念视频
Neural Regulation
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
Long-term Potentiation
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Long-term Potentiation
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when presynaptic neurons...
Hebbian LTP
LTP can occur when presynaptic neurons...
Ligand-gated Ion Channels
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
GPCRs Regulate Adenylyl Cylase Activity
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
Two...
Two...
Ligand-Gated Ion Channel Receptor: Gating Mechanism
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
