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两个分子多重综合症综合征面板的比较评价与急性胃肠炎

Kuenyoul Park¹, Bo-Moon Shin¹

¹Department of Laboratory Medicine, Sanggye Paik Hospital, School of Medicine, Inje University, 1342, Dongil-ro, Nowon-gu, Seoul 01757, Korea.

Diagnostic microbiology and infectious disease

|March 6, 2024

概括

Allplex和Seeplex测定显示了对检测急性胃肠炎病原体的高度一致. 奥尔普莱克斯在识别沙门氏菌和致病性大肠杆菌等细菌标方面表现出卓越的灵敏度.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
分子诊断学分子诊断

背景情况:

急性胃肠炎 (AGE) 是一个重大的公共卫生问题.
准确和快速的病原体识别对于有效管理AGE至关重要.
分子诊断试验比传统的病原体检测方法具有潜在的优势.

研究的目的:

为了比较两种多重分子试验的性能,Allplex Gastrointestinal V/B1/B2和Seeplex Diarrhea V/B1/B2 ACE,用于检测AGE患者的常见肠道病原体.
评估整体百分比协议 (OPA) 并确定两种测试之间的特定病原体检测差异.

主要方法:

进行了一项比较研究,使用了来自AGE患者的432个临床标本.
使用Allplex胃肠道V/B1/B2试验和Seeplex腹V/B1/B2ACE试验并行检测病毒和细菌标.
进行了统计分析,以确定总体百分比的一致性,并确定病原体检测的显著差异.

主要成果:

两种测定都显示出高的总体百分比一致 (>95%),其中Clostridium difficile的OPA最低为95.4%,达到95.4%.
与Seeplex (48.8%) (P = 0.002) 相比,Allplex检测到任何细菌或病毒点的样本中阳性的比例更高 (54.9%).
阿尔普莱克斯对沙门氏菌种类表现出更高的敏感性. (40对27个样本) 和致病性大肠杆菌 (40对未说明的样本),而Seeplex检测到更多的Clostridium perfringens (70对未说明的样本). 西格拉菌种类. 通过两种分子测定检测到,但不是通过培养.

结论:

Allplex和Seeplex测定是诊断AGE的可靠工具,具有很高的总体一致性.
Allplex测试显示对关键细菌病原体,包括沙门氏菌的增强敏感性. 和致病性大肠杆菌,可能提供更全面的诊断信息.
分子测试对于识别AGE中的肠道病原体是有价值的,不同微生物的具体测试性能不同.

关键词:

剧烈的胃肠炎是一种急性胃肠炎.腹腹腹腹腹腹腹腹腹肠道病原体肠道病原体多重复合PCR是一种多重复合PCR.核酸技术的核酸技术是什么综合征测试是为了测试综合征.

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