相关实验视频

Updated: Jul 1, 2025

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Published on: July 2, 2020

人类iPSC衍生型细胞样细胞的转录形状,这些细胞被暴露在一组异生菌中

Cormac Murphy¹, Paul Jennings¹, Anja Wilmes¹

¹Division of Molecular and Computational Toxicology, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam Institute of Molecular and Life Sciences (AIMMS), Faculty of Science, Vrije Universiteit, Amsterdam, the Netherlands.

Toxicology in vitro : an international journal published in association with BIBRA

|March 6, 2024

概括

诱导的多能干细胞衍生的 podocytes 揭示了从质毒素的转录变化. 这种模型对调查病机制和药物毒性的研究充满希望.

科学领域:

腎臟病學 (nephrology) 是一種醫學.
毒理学毒理学毒理学
干细胞生物学干细胞生物学

背景情况:

足细胞对于膜过至关重要;它们的有限增殖给体外研究带来了挑战.
诱导多能干细胞 (iPSCs) 提供了一种可再生的来源,用于生成类似细胞的细胞.
了解细胞损伤是预防慢性病进展的关键.

研究的目的:

评估已知质毒素和其他化合物的对iPSC衍生小细胞的转录效应.
评估iPSC衍生的 podocytes作为毒理学和机理学研究的模型的实用性.

主要方法:

暴露iPSC衍生的受体细胞在24小时内接触8种化合物.
转录组分析 (RNA测序) 用于识别基因表达变化.
途径分析以了解毒性的分子机制.

主要成果:

多克索鲁比辛,帕米德罗纳酸,甲和环素A显著改变了细胞转录组.
甘他素,化和瓦尔酸具有较轻微的转录基因影响;布苏尔方没有影响.
获得了机理性见解,包括p53通路参与 (多克索鲁比辛),HIF1alpha激活 (帕米德罗酸盐) 和氧化应激 (甲).

结论:

从iPSC衍生出来的细胞是研究化合物对球细胞的毒理作用的有价值模型.
转录形状分析揭示了不同毒素影响的特定途径,有助于理解损伤机制.
这种方法支持对病的预测毒理学和机制学研究的发展.

关键词:

德克索鲁比辛是一种多克索鲁比辛.帕米德罗酸盐是一种波多细胞 (Podocytes) 是一种细胞.有毒性有毒性文字转录学 (Transcriptomics) 是一个学科.这些是iPSCs.

更多相关视频

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies

Published on: May 26, 2023

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

相关概念视频

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.