暴露在固体表面冷和玻璃化中的水滴的冷却动态
Dejia Liu1, Harriëtte Oldenhof1, Xing Luo2
1Biostabilization Laboratory - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany; Unit for Reproductive Medicine - Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany.
Cryobiology
|March 6, 2024
概括
固体表面玻璃化 (SSV) 和冷 (SSF) 提高了冷保存的冷却速度. 糖溶液冷却速度比DMSO更快,在低度下降速率更高,而40%的糖显示红细胞冷保存效果比DMSO更好.
科学领域:
- 低温生物学 低温生物学
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
背景情况:
- 固体表面冷/玻璃化 (SSF/SSV) 提供比液浸泡更高的冷却速度,用于冷保存.
- 这种方法涉及将样本滴滴沉积在预冷的金属表面上.
- 达到更高的冷却速度可以提高冷生存率.
研究的目的:
- 在SSF/SSV过程中数值模拟冷却剂 (CPA) 溶液的冷却动态.
- 为了研究CPA类型 (糖醇与DMSO),度和滴滴大小对冷却速度的影响.
- 实验验证模拟结果并评估人类红细胞 (RBC) 的冷保存结果.
主要方法:
- 数字模拟滴滴冷却动态,建模圆柱形底部和半圆形顶部形状.
- 利用了材料特性 (密度,热容量,导热性) 的文献数据,使用多项式拟合进行了推断.
- 实验性冷保存人类红细胞使用SSV/SSF与不同的CPA类型和度.
主要成果:
- 与二甲基硫氧化物 (DMSO) 溶液相比,甘油 (GLY) 溶液的冷却速度更快.
- 冷却速度随着CPA度的下降而增加.
- 在40% (w/w) 的CPA下,糖醇显示红细胞的冷保存优于DMSO,由较低的血液溶解率表明.
结论:
- 在SSF/SSV中的冷却动态受到CPA类型和度的显著影响.
- 甘油在较高度下对红细胞冷保存具有与DMSO相比的潜在优势.
- 数字模拟为优化SSF/SSV协议提供了宝贵的见解,以提高冷保存结果.
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