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纤维素 1 支持猪体内的卵细胞成熟

Mingju Sun¹, Xiangyu Wang¹, Fanglong Bi¹

¹College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.

International journal of biological macromolecules

|March 6, 2024

概括

毛囊液中的纤维素1 (FN1) 显著增强了卵细胞成熟和胚胎发育. 这种蛋白质通过激活PI3K信号通路来改善卵细胞质量,为体外成熟提供了新的见解.

科学领域:

生殖生物学生殖生物学
细胞和分子生物学细胞和分子生物学
生物化学生物化学

背景情况:

卵细胞体外成熟 (IVM) 旨在模仿体内卵泡液 (FF) 环境,以获得更好的结果.
在FF中调节卵细胞成熟的关键因素在很大程度上仍未确定.
了解这些因素对于优化辅助生殖技术至关重要.

研究的目的:

识别和描述调节卵细胞成熟的FF因素.
阐明分子机制,通过确定因素提高卵细胞的质量和发育.
为了研究纤维素1 (FN1) 在卵细胞体外成熟中的作用.

主要方法:

蛋白质组分析以预测FF的潜在监管因素.
在试验室中成熟的卵细胞具有不同度的Fibronectin 1 (FN1).
评估卵细胞成熟 (极体挤出),胚胎发育,形态,DNA完整性 (γ-H2AX),氧化应激 (ROS),亡,谷氨 (GSH) 和线粒体含量.
分析关键信号通路组件,包括PI3K信号.

主要成果:

毛囊液 (FF) 和血清显著促进卵细胞成熟和胚胎发育,FF更有效.
纤维素1 (FN1) 被确定为一个关键因素;其管理改善了卵细胞极体挤出 (PBE) 率.
FN1治疗增强了形态,减少了DNA损伤和亡,增加了抗氧化剂和线粒体含量,并激活了PI3K信号通路.

结论:

纤维素1 (FN1) 是毛囊液中的一个重要因素,增强了卵细胞的成熟.
FN1可以改善卵细胞的核和细胞质成熟.
该机制涉及PI3K信号通路激活的促进.

关键词:

纤维蛋白 1 纤维蛋白 1 在体外成熟.卵子细胞是卵子细胞.

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