人体脏移植中的组织内存T细胞具有全活性潜力
Daphne M Hullegie-Peelen1, Hector Tejeda-Mora1, Marjolein Dieterich1
1Erasmus Medical Center Transplant Institute, Department of Internal Medicine, Nephrology and Transplantation, University Medical Center Rotterdam, Rotterdam, the Netherlands.
概括
脏移植中的组织居民记忆T (TRM) 细胞可以全活性. 这些细胞在移植前在患者的血液中被发现,在移植后的脏中发现了接受者和供体细胞.
科学领域:
- 免疫学 免疫学 免疫学
- 移植生物学 移植生物学
- 细胞免疫学 细胞免疫学
背景情况:
- 组织内存T (TRM) 细胞在移植排斥中的作用尚未完全理解.
- 研究TRM细胞的全活性对于改善移植结果至关重要.
研究的目的:
- 为了确定移植中TRM细胞的全活性潜力.
- 从外围血液中追踪先前存在的全活性T细胞克隆到脏的探索者.
主要方法:
- 在移植前血液中通过混合淋巴细胞反应识别出全活性T细胞受体 (TCR) 克隆.
- 使用单细胞RNA和TCR测序对受体T细胞和扩增T细胞.
- 在探器中,在接受者和捐赠者TRM细胞内追踪鉴定出全活性TCR克隆.
主要成果:
- 在探器中,在0%至9%的受体衍生的TRM细胞中检测到全活性TCR.
- 主要在CD4+和CD8+效应体TRM细胞中发现了全活性TCRs.
- 在多达4%的来自捐赠者的TRM细胞中也发现了全反应性TCR,这表明自我反应性.
结论:
- 周围血液中具有全活性潜力的T细胞可以透移植.
- 这些透的T细胞可以分化为组织内存T细胞.
- 全活性和潜在的自我反应性TRM细胞的存在突显了移植排斥的新机制.
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