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新型生物传感器用于高通量检测与孕激素受体相互作用的内分泌干扰剂

Diana A Stavreva¹, Lyuba Varticovski², Razi Raziuddin²

¹Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, 41 Medlars Dr., Bethesda, MD, 20892-5055, USA. stavrevd@mail.nih.gov.

Scientific reports

|March 6, 2024

概括

孕激素受体 (PR) 相互作用化合物的环境污染对健康构成风险. 一个新的生物传感器可以在环境样本中快速检测这些内分泌干扰化学物质 (EDC).

科学领域:

环境科学环境科学
毒理学毒理学毒理学
生物技术是生物技术.

背景情况:

孕激素受体 (PR) 相互作用化合物与严重的健康问题有关.
目前用于检测这些环境污染物的方法通常是复杂和耗时的.

研究的目的:

开发一种灵敏,基于活动的生物传感器,用于快速,可靠地选与PR相互作用的内分泌干扰化学物质 (EDC).
为了能够有效地识别新型PR配体和环境污染物.

主要方法:

设计了一种基于细胞的新型生物传感器,表达核mCherry-NF1和GFP-标记的嵌合体 (GFP-GR-PR) 的葡萄糖皮质体受体 (GR) N末端融合到PR连接体结合域 (LBD).
这种生物传感器检测到PRA和PRB异型体的PR激活.
作为对PR激动剂/对抗剂的反应,奇梅拉从细胞质转移到细胞核,可实现实时,度和时间依赖的监测.

主要成果:

生物传感器在检测PR相互作用化合物方面表现出灵敏度和可靠性.
在活细胞中加入核NF1标签简化了选过程,消除了细胞固定和核染色的需要.
河流水样本的初步选显示,与PR相互作用的污染物存在广泛的低水平污染.

结论:

开发的生物传感器为在环境矩阵中快速有效选与PR相互作用的EDC提供了显著的进步.
这种工具可以促进发现新的PR配体,并识别环境污染物.
在所有测试的河水样本中存在与PR相互作用的污染物,突显了持续环境监测和风险评估的必要性.

关键词:

生物活动是生物活动.激素这些激素.河水河水河水河水查检查查检查查检查

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