一种超敏感和高通量捕捉微LC-MS的方法,用于量化循环维生素D代谢物,并应用于多发性硬化症患者
Flora Qu1,2, Ming Zhang2, Bianca Weinstock-Guttman3
1Department of Biochemistry, University at Buffalo, State University of New York, Buffalo, NY, USA.
Scientific reports
|March 6, 2024
概括
这项研究提出了一种新的LC-MS方法,用于精确测量维生素D (VitD) 代谢物. 在多发性硬化症患者中,先进的技术揭示了25(OH) D2和D3代谢物之间的反向关系.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 临床化学 临床化学
背景情况:
- 准确量化维生素D (VitD) 代谢物对于理解生理和病理过程至关重要.
- 现有的液体染色体-并联质谱法 (LC-MS) 方法在敏感性,准确性,吞吐量和低丰度VitD代谢物的稳定性方面扎.
研究的目的:
- 开发一种超灵敏,强大和高通量方法,用于量化血清中的VitD代谢物.
- 应用这种新的方法来研究多发性硬化症患者的VitD代谢物水平.
主要方法:
- 开发了一种新的管道,将捕获微LC (T-μLC) 与狭窄窗口隔离选择反应监测MS (NWI-SRM) 结合起来.
- 该方法使用选择性捕获和传递方法来去除矩阵组件并提高灵敏度.
- 在血清样本分析之前,已经采用了衍生法.
主要成果:
- 与现有的LC-MS方法相比,开发的方法实现了显著较低的量化极限 (LOQ).
- 1,25(OH) 2D3的LOQ为1.0 pg/mL,24,25(OH) 2D3的LOQ为5.0 pg/mL,25(OH) D2和25(OH) D3的LOQ为30 pg/mL,所有这些都在9分钟周期内.
- 对218名多发性硬化症患者的分析显示,25(OH) D2和D3代谢物之间的负相关性 (r = -0.44到 -0.51).
结论:
- 新的T-μLC-NWI-SRM管道提供了对VitD代谢物的超敏感,强大和高吞吐量量化.
- 这种方法可以精确分析VitD代谢物,这对于临床研究至关重要.
- 该研究在多发性硬化症患者中发现了特定的VitD代谢物之间的显著负相关性,需要进一步调查.
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