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相关概念视频

Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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Protein Complex Assembly02:41

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
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The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
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Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
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相关实验视频

Updated: Jul 1, 2025

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
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通过JADE子单元指导HBO1复杂的功能.

Nitika Gaurav1, Akinori Kanai2, Catherine Lachance3

  • 1Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, USA.

Nature structural & molecular biology
|March 6, 2024
PubMed
概括
此摘要是机器生成的。

JADE 蛋白子单元对于 HBO1 综合体至关重要.

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科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • HBO1乙转移酶复合体在调节基因转录和表观遗传程序方面发挥着至关重要的作用.
  • 玉是HBO1复合体的一个关键子单元,对其功能至关重要.
  • 了解JADE的机制对于破译HBO1复杂活性至关重要.

研究的目的:

  • 阐明JADE促进HBO1复合体对染色质的招募的机制.
  • 研究JADE如何调解HBO1复合体的酶活性.
  • 探索JADE在白血病发生和病理过程中的作用.

主要方法:

  • 使用了结构性,基因组学和体内复杂组装研究.
  • 对JADE的PHD1--结-PHD2 (PZP) 域进行了分析,以确定核细胞参与.
  • 评估了对Histone H3K4甲基化状态及其对HBO1复合体活性的影响.

主要成果:

  • JADE子单元的PZP域与基因素H3和DNA结合,使染色体结合成为可能.
  • 非甲基化H3K4将HBO1复合物的活性转向向希斯H4乙化.
  • H3K4高甲基化改变了HBO1复合物的基质选择性.
  • 在白血病发生过程中,NUP98-JADE2融合的转化活性被JADE的PZP域增强.

结论:

  • JADE对于 HBO1 综合体的基因组招募和酶功能至关重要.
  • JADE与核细胞的相互作用决定了基质特异性和表观遗传调节.
  • 在HBO1复合体的病理活动中,包括白血病发生,JADE起着重要的作用.