在鉴定UDP-Glycosyltransferases的进步,为金氏酸生物合成
Xiaoxuan Yuan1, Ruiqiong Li2, Weishen He3
1Institute of Structural Pharmacology & TCM Chemical Biology, College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China.
Journal of natural products
|March 7, 2024
概括
帕纳克斯植物中的关键化合物金赛诺化物具有药用益处,但很难提取. 本研究探讨在微生物中使用葡萄糖转移酶 (UGTs) 通过异构生物合成进行高效,大规模的金氏化物生产.
科学领域:
- 自然产品生物合成 自然产品生物合成
- 代谢工程是代谢工程.
- 酶学 是一种酶学.
背景情况:
- 来自帕纳克斯 (Panax) 属的金赛诺化物具有显著的药用特性,包括抗心血管,细胞毒性,抗衰老和抗糖尿病作用.
- 低自然度和提取困难限制了金氏的应用,需要使用替代生产方法.
- 异质生物合成为这些有价值的自然产品的有针对性的生产提供了一个有希望的途径.
研究的目的:
- 审查尿素二酸盐依赖的糖系转移酶 (UGTs) 的鉴定和功能特征,这些酶参与了人参酸生物合成.
- 为了突出工程微生物细胞工厂的大规模生产的进步.
- 讨论UGT采矿的策略和潜在的快速选方法,以发现新型的甘氨基转移酶.
主要方法:
- 在植物 (如Panax人参) 和微生物 (如Bacillus subtilis,Saccharomyces cerevisiae) 中对UGT鉴定和功能研究的文献综述.
- 对与UGT相关的用于生化生产的微生物细胞工厂的分析.
- 探索UGT采矿策略,包括快速选和识别技术.
主要成果:
- 对金氏酸甘氨酸骨架的生物合成途径的成功解码.
- 在识别和研究负责各种生物体中银酸糖化酶的UGT方面取得了重大进展.
- 开发微生物细胞工厂,以提高人参化物生产.
结论:
- UGTs对于金氏的结构多样性和药理活性至关重要.
- 异质生物合成,特别是利用工程微生物系统,是可扩展的金氏化物生产的可行策略.
- 对UGT采矿的进一步研究将有助于发现用于生产罕见人参酸的新型酶.
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