酸对Issatchenkia terricola WJL-G4细胞膜结构和功能的影响
Xiangfeng Meng1, Xinyi Liu1, Yihong Bao1,2
1College of Life Science, Northeast Forestry University, No. 26, Hexing St., Harbin 150040, China.
Journal of applied microbiology
|March 7, 2024
概括
在酸压力下,Issatchenkia terricola调整其细胞膜结构和功能. 高度 (80g/L以上) 显著改变膜特性,可能降低酵母的功能.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 酸是一种常见的工业化学品.
- 了解酵母细胞膜对环境压力因素的反应对于工业应用至关重要.
研究的目的:
- 为了研究酸对Issatchenkia terricola的细胞膜结构和功能的影响.
- 为了确定I. terricola对酸暴露的耐受性极限.
主要方法:
- 生理学分析包括化染,微生物对碳化合物的粘附性测试,传输电子显微镜,光异性,流细胞计和气色谱-质谱.
- 评估膜完整性,疏水性,流动性,亡和脂肪酸甲基组成.
主要成果:
- 酸度的增加降低了细胞活力,膜完整性和流动性.
- 随着酸水平的提高,亡率,膜透性,水性和厄戈斯特醇含量显著增加.
- 酶活动 (Na+,K+-ATPase,Ca2+,Mg2+-ATPase) 增加,但细胞结构在80g/L酸下受到损害.
结论:
- 伊萨切肯基亚 (Issatchenkia terricola) 可以在酸度低于60g/L的情况下适应其膜结构和功能.
- 80g/L以上的度会导致剧烈的变化,可能会损害酵母的功能.
更多相关视频
11:47Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function
Published on: January 22, 2017
10.6K
17:35A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
Published on: November 1, 2013
40.0K
相关概念视频
The Citric Acid Cycle: Overview
17.3K
In aerobic organisms, the citric acid cycle is the second stage of cellular respiration wherein molecules derived from the breakdown of carbohydrates, proteins, and fats are oxidized into carbon dioxide and energy. This process is also known as the tricarboxylic acid (TCA) cycle as the first product of the cycle, citric acid, contains three carboxyl groups in its structure. Alternatively, this cycle is also referred to as the Krebs cycle, in honor of its discoverer Sir Hans Krebs.
The citric...
The citric...
17.3K
Assembly of the Lipid Bilayer in the ER
3.2K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.2K
