用负控对 ForenSeq Kintelligence 库进行多重复合的影响和考虑因素
Gunmeet Kaur Bali1, Daniela Cuenca1, Jeanette Wallin1
1California Department of Justice, Jan Bashinski DNA Laboratory, Richmond, California, USA.
Electrophoresis
|March 7, 2024
概括
在每个法医大规模并行测序 (MPS) 运行中包括负控制,消耗了宝贵的测序空间,可能导致等位基因脱落,并在遗传谱系数据库中增加未知的关联.
科学领域:
- 法医科学 法医科学 法医科学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 负控制对于监测法医DNA分析中的污染至关重要.
- 大规模并行测序 (MPS) 在法医实验室越来越多地被采用.
- 目前的FBI质量保证标准要求在法医DNA分析中进行负控制.
研究的目的:
- 通过使用ForenSeq Kintelligence Kit,评估在每个测序运行中包含负控制的必要性和影响.
- 为了评估负控在后续序列运行中的实用性,在初始库准备后运行.
主要方法:
- 研究了在每个 ForenSeq Kintelligence 测序运行中包含负放大控制的效果.
- 量化了负控库所占的测序空间.
- 在GEDmatch PRO中分析了对含DNA样本的影响,包括等位基因/位点脱落和未知的关联.
主要成果:
- 负控制库占据了总测序运行输出的7% - 14%.
- 在某些情况下,减少DNA样本的测序空间导致了等位基因或位点脱落.
- 在GEDmatch PRO中观察到第六到第七次未知关联的情况增加.
结论:
- 在ForenSeq Kintelligence Kit的每个测序运行中,在初始库准备后,常规包含负控并非必不可少.
- 这种做法消耗了大量的测序资源,可能会损害对有价值的法医DNA样本的分析.
- 优化负控制的使用可以提高法医遗传谱系中的MPS效率和数据质量.
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