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Updated: Jul 1, 2025

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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
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纳米石墨烯氧化物颗粒通过参与卵细胞成熟的改变基因表达诱导遗传异常
Sreelakshmi Krishnakumar1, Raghunath Nair Malavika1, Shantikumar V Nair1
1School of Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi Campus, Kerala, India.
Nanotoxicology
|March 7, 2024
概括
暴露于纳米石墨烯氧化物颗粒 (nGO) 影响脊椎动物的生殖线发展和几代人的生育能力. 虽然较高剂量导致异常,但较低剂量对生殖线没有不良影响,这表明nGO暴露的安全门.
科学领域:
- 环境毒理学环境毒理学
- 纳米粒子研究的研究.
- 生殖生物学 生殖生物学
背景情况:
- 石墨烯氧化物纳米颗粒 (GONP) 对脊椎动物生殖系的长期影响基本上是未知的.
- 了解这些影响对于评估与纳米材料相关的环境和健康风险至关重要.
研究的目的:
- 为了研究纳米石墨烯氧化物颗粒 (nGO) 对生殖细胞发育和生殖结果的跨代影响,在日本 medaka.
- 为了确定暴露时间在关键性雌同体发育阶段对遗传影响的影响.
主要方法:
- 在不同发育阶段,日本medaka胚胎和成年人暴露于合成的nGO (300-600nm,-36.8mV表面电荷).
- 对暴露个体及其F1和F2代的生物相容性,生殖细胞数量,生育能力和基因表达进行了分析.
主要成果:
- 暴露于nGO导致胚胎致死率超过1μg/ml,并在次致死剂量时增加生殖细胞数量.
- 长期暴露导致F1和F2世代的生育能力下降,影响在10μg/ml时更为明显.
- 与卵细胞成熟相关的基因表达在随后的几代人中发生了变化,遗传模式因暴露时间而异.
结论:
- 纳米石墨烯氧化物颗粒可以诱导脊椎动物的跨代生殖毒性.
- 在生殖细胞发育过程中暴露时间显著影响nGO的遗传效应.
- 较低的nGO剂量 (1μg/ml) 似乎对生殖线是安全的,但较高的剂量需要谨慎.
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