慢性压力改变了肝脏代谢和热力学呼吸效率,影响了C57BL/6小鼠的表观遗传学
Aleksandra Nikolic1,2, Pia Fahlbusch1,2, Nele-Kathrien Riffelmann1
1Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Duesseldorf, Leibniz Center for Diabetes Research, 40225 Duesseldorf, Germany.
iScience
|March 7, 2024
概括
慢性压力会破坏肝脏代谢和线粒体功能,导致持久的表观遗传变化,甚至在压力恢复后也会损害能量平衡. 这会影响代谢疾病的风险.
科学领域:
- 代谢研究的研究.
- 线粒体生物学 线粒体生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 慢性压力与增加代谢疾病风险有关.
- 持续的压力可能会改变肝脏代谢重编程和线粒体功能.
研究的目的:
- 研究慢性可变压力 (Cvs) 对小鼠肝脏能量代谢和线粒体功能的影响.
- 探索这些代谢变化背后的潜在表观遗传机制.
主要方法:
- 雄性C57BL/6小鼠受到慢性变量压力 (Cvs).
- 测量了能量消耗,体重,葡萄糖代谢和脂质氧化.
- 评估了线粒体功能,包括复合I效率.
- 在压力和恢复期间分析了DNA甲基化模式和单碳代谢.
主要成果:
- Cvs减少了能量消耗和体重,改变了葡萄糖代谢和增加了脂质氧化.
- 线粒体综合体I显示热力学效率提高,与血糖相关,与能量消耗相反.
- 在心血管复苏后,代谢灵活性会丧失.
- 在恢复过程中出现了表观遗传变化,包括线粒体DNA甲基化和改变的转录网络.
结论:
- 慢性变量压力迅速重新编程肝脏的能量代谢.
- 持久的表观遗传修饰,特别是在线粒体DNA中,有助于压力后的代谢功能障碍.
- 这些变化可能是慢性压力后代谢疾病风险增加的基础.
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