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土壤和种子都影响大麻种子内层微生物群中的细菌多样性

Christopher R Dumigan¹, Michael K Deyholos¹

¹Department of Biology, Faculty of Science, University of British Columbia, Kelowna, BC, Canada.

Frontiers in plant science

|March 7, 2024

概括

大麻微生物组是由种子和土壤共同塑造的. 种子衍生微生物被保护,在植物健康中发挥关键作用,影响营养循环和激素调节.

科学领域:

微生物学微生物学
植物科学植物科学
遗传学是一个遗传学.

背景情况:

植物生物组显著影响植物健康.
由于新的种植和繁殖方法 (克隆和种子),大麻sativa微生物群引起了人们的兴趣.
雄性体从它们的母植物中获得种子载体内体.

研究的目的:

为了研究种子和土壤衍生的细菌对大麻苗的内层的影响.
为了比较大麻和药物类型大麻的基因型.
了解种子所携带的微生物在大麻中的作用.

主要方法:

使用三种大麻基因型和两种土壤来源 (消毒和未消毒) 的多因素元基因组研究.
在表面灭菌后,16S rDNA amplicon对幼苗内细胞进行测序.
使用Qiime2进行多样性分析 (alpha和beta),使用PiCRUST2.2进行功能推理.

主要成果:

土壤显著影响了内生细菌的α-多样性,β-多样性,相对丰富性和功能基因.
大麻基因型对β多样性有显著影响,特别是在无菌土壤中.
甲基蛋白细菌和细菌是所有条件下最丰富的种类.

结论:

大麻幼苗内层微生物组的一部分是种子衍生和保存的.
种子携带的内细胞有助于营养循环,激素调节和抗生素生产.
种子和土壤都对塑造生长大麻苗的微生物组至关重要.

关键词:

它们的内基质 (endophyte) 是:大麻是一种麻.甲基基因组 (metagenome) 是一个基因组.我们的微生物群.植物生物组 (phytobiome) 是一个生物组.

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