相关实验视频

Updated: Jul 21, 2026

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Published on: October 25, 2011

乳腺癌中ki-67测试:通过评分方法和样本类型评估可变性,以及对治疗资格的潜在后续影响

Therese Bocklage¹, Virgilius Cornea¹, Caylin Hickey¹

¹Departments of Pathology and Laboratory Medicine.

Applied immunohistochemistry & molecular morphology : AIMM

|March 7, 2024

概括

这项研究突出了Abemaciclib资格的Ki-67评分的显著差异. 精细的Ki-67评估方法对于减少变异性和确保准确的患者选择ER阳性/HER2阴性乳腺癌治疗至关重要.

科学领域:

在瘤学瘤学.
病理学病理学病理学
生物标记分析生物标记分析

背景情况:

阿贝马西克利布已批准用于治疗ER阳性/HER2阴性乳腺癌,基基67≥20%.
缺乏对Ki-67测试和评分方法的标准化指南,造成了模两可.
患者接受abemaciclib治疗的资格可能会受到评分变化的影响.

研究的目的:

评估不同样本类型和评分方法中Ki-67表达的变异性.
将手动Ki-67评分技术 (估计,未加权,加权) 与定量图像分析 (QIA) 相比较.
评估评分变化的影响,以确定对abemaciclib治疗的资格.

主要方法:

从50名符合abemaciclib.lib.条件的患者获得的乳房样本的全面分析.
三位病理学家使用估计 (EST),未加权 (UNW) 和加权 (WT) 手动评分方法进行了盲目审查.
使用HALO平台的定量图像分析 (QIA) 用于比较.
在核心针活检 (CNB),切除 (RES) 和带淋巴结转移 (ALN) 样本中评估Ki-67表达.

主要成果:

平均Ki-67表达因得分方法而异:EST (14.68%),UNW (14.46%),WT (14.15%) 和QIA (11.15%). 这两种表达方式均有所不同.
与EST (ICC1=0.922) 和UNW (ICC1=0.949) 相比,加权 (WT) 方法显示了较低的观察者间变异性 (ICC1=0.959).
基于Ki-67 ≥20%的治疗资格范围从24% (QIA) 到40% (EST),表明存在显著差异.
在CNB,RES和ALN标本中没有发现Ki-67表达的统计学上显著差异.

结论:

在Ki-67评分中存在显著的临床变化,以确定abemaciclib的资格.
权重评分方法和QIA显示了减少观察者间变异性的潜力.
进一步完善Ki-67评分方法是必不可少的,以确保准确的患者选择针对性乳腺癌治疗.

更多相关视频

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

相关概念视频

Nursing Assessment of the Genitourinary System II: Inspection and Palpation

Nursing Assessment of the Genitourinary System II: Inspection and Palpation

The nursing assessment of the genitourinary (GU) system involves a systematic inspection and palpation to identify abnormalities in the kidneys, bladder, and surrounding structures.InspectionMouth: Inspect for signs of kidney dysfunction, such as stomatitis (inflammation of the mouth) and ammonia breath, which may occur in advanced kidney disease due to the buildup of urea, breaking down into ammonia.Skin: Check for pallor, which could indicate anemia caused by kidney disease. Look for...

Kidney Transplant I: Introduction

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...

Kidney Transplant III: Nursing Management

Kidney Transplant III: Nursing Management

Postoperative Nursing Management for Kidney Transplant PatientsPostoperative nursing management care includes monitoring the surgical site, encouraging early movement, and promoting lung health through breathing exercises. Nurses also administer prescribed medications like H2-blockers, such as famotidine, or proton pump inhibitors, like omeprazole, to help prevent gastrointestinal ulcers and bleeding. Fungal infections in the mouth and bladder can result from immunosuppressive and antibiotic...

Imaging Studies I: Kidney, Ureter, and Bladder Studies

Imaging Studies I: Kidney, Ureter, and Bladder Studies

Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...

Chronic Kidney Disease III: Interprofessional Care

Chronic Kidney Disease III: Interprofessional Care

Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...