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量化精子DNA碎片化与辅助生殖技术结果的关联:一个总体审查

Hassan Maghraby^1,2, Mohamad AlaaEldein Elsuity^2,3,4, Nehal Adel^2,5

¹Obstetrics and Gynaecology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

BJOG : an international journal of obstetrics and gynaecology

|March 7, 2024

概括

这一综合性审查没有发现有说服力的证据将精子DNA碎片化 (SDF) 与辅助生殖技术 (ART) 的结果联系起来. 建议对有关SDF的索赔保持谨慎.

科学领域:

生殖医学生殖医学
遗传学遗传学是一个
证据综合证据综合

背景情况:

系统审查和元分析对于临床实践至关重要,但可能会受到偏见和差异的影响.
总体审查综合现有审查以评估证据质量,特别适用于关于精子DNA碎片化 (SDF) 和辅助受孕结果的相互矛盾的发现.
关于SDF和辅助生殖技术 (ART) 成功率之间的因果关系存在分歧的文献.

研究的目的:

进行综合性审查,综合系统性审查和对SDF和ART结果之间关联的元分析的证据.
评估SDF对生育治疗的影响现有研究的质量和一致性.

主要方法:

进行全面的数据库搜索 (PubMed,Scopus,Cochrane Library,Web of Science,Embase) 以对SDF和ART结果进行系统审查和元分析.
使用AMSTAR 2和ROBIS进行包含的审查的质量评估,并使用纠正覆盖面积 (CCA) 进行研究重叠分析.
对关键ART结果 (活产,怀孕,流产等) 的效果大小 (odds比率) 的重新估计. 和证据强度的分类.

主要成果:

包括22个系统性审查 (15个元分析),显示初级研究中适度到高度重叠.
AMSTAR 2和ROBIS的评估表明,在包括的审查中,质量极低至低,偏差风险很高.
重新评估的分析显示,SDF与各种ART结果之间存在弱或无意义的关联,包括活产和怀孕率.

结论:

这次综合性审查没有发现有说服力或高度暗示性证据将SDF与ART结果联系起来.
现有审查中的质量和偏见风险限制了关于SDF对生育影响的结论的强度.
在辅助受孕的背景下,在提出有关SDF的索赔,政策或建议时要谨慎.

关键词:

DNA碎片化指数 (DFI) 是一个指数.辅助生殖技术 (ART) 是一种助孕技术.细胞体内精子注射 (ICSI) 是一种精子注射方法.在体外受精 (IVF)精子DNA碎片化 (SDF) 的方法

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