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泰国中的西红死环斑病毒的完整基因组序列来自下一代测序

Phoowanarth Maneechoat¹, Pissawan Chiemsombat², Salvador Lopez³

¹Plant Protection Research and Development Office, Department of Agriculture, Ministry of Agriculture and Cooperatives, 50 Phaholyothin Road, Lat Yao, Chatuchak, Bangkok, 10900, Thailand.

Archives of virology

|March 7, 2024

概括

番茄死环点病毒 (TNRV) 严重影响泰国的胡和番茄作物. 这项研究详细介绍了TNRV分离体的完整基因组序列,揭示了它与其他病毒的遗传关系.

科学领域:

植物病毒学植物病毒学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

番茄死环斑病毒 (TNRV) 在泰国胡和番茄作物中造成显著的产量和质量损失.
2011年在泰国首次发现TNRV,对农业构成持续威胁.

研究的目的:

为了确定从中分离的TNRV的完整基因组序列.
分析这种TNRV分离物与其他已知的病毒的遗传关系.

主要方法:

使用下一代测序来获得TNRV分离-CR.的完整基因组序列.
进行了基因组RNA段的核酸和氨基酸序列的比较分析.
用单个和连接的基因组RNA序列进行了遗传学分析.

主要成果:

TNRV分离-CR的完整基因组由三个RNA段 (L,M,S) 组成,总长度为16595个核酸.
该基因组编码了五种典型的orthootospovirus蛋白质:L,NSm,GNGc,NSs和N.
TNRV-CR与胡菌斑点病毒 (PCSV) 共享73.6-82.3%的核酸和81.1-91.9%的氨基酸序列相同性.
遗传学分析始终将TNRV与PCSV聚合在一起,支持TNRV作为一个独特的ortotospovirus.

结论:

完整的基因组序列为了解TNRV生物学及其对作物的影响提供了基础.
证实TNRV是一种独特的orthootospovirus,与PCSV密切相关.
对连接的ortotospovirus基因组序列的分析对未来在这个病毒群体内的遗传学研究有价值.

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