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在发酵香肠中选择性抑制金黄色葡萄球菌的细菌素的基因组识别和表征
Hongbiao Li1, Yongqi Yang1, Lanxin Li1
1College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
Probiotics and antimicrobial proteins
|March 7, 2024
概括
乳植物菌植物SL47产生细菌素SL47,是一种强大的生物防腐剂. 这种细菌素表现出广泛的抗菌活性,并降低了发酵香肠中的金色葡萄球菌,突出了其在肉制品中的潜力.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
背景情况:
- 生产细菌素的乳酸细菌对于发酵食品的保存至关重要.
- 乳植物菌植物SL47从发酵的香肠中分离出来.
- 新型细菌素为化学防腐剂提供了替代品.
研究的目的:
- 从Lactiplantibacillus plantarum SL47.7.中分离和描述细菌素SL47的细菌素.
- 评估细菌素SL47.7的抗菌谱和作用机制.
- 评估L. plantarum SL47和细菌素SL47作为发酵香肠中的生物防腐剂的有效性.
主要方法:
- 使用乙烯酸乙烯,Sephadex G-25凝染色学和RP-HPLC,分离和净化细菌素SL47.
- 通过HPLC-MS/MS和全基因组测序识别细菌素SL47.
- 细菌素SL47的稳定性,抗菌谱和作用机制的表征,包括对金黄色葡萄球菌的影响.
主要成果:
- 细菌素SL47包括植物素A,J,K和N.
- 细菌素SL47具有高度的热稳定性,pH稳定性,并表现出广泛的抗菌活性.
- 用细菌素SL47的治疗诱导了金黄色葡萄球菌的亡,并显著减少了其在发酵香肠中的数量.
结论:
- 乳植物菌植物菌SL47及其细菌素SL47显示出作为天然生物防腐剂的巨大潜力.
- 细菌素SL47的稳定性和广泛的活性使其适用于肉制品应用.
- 对细菌素SL47的进一步研究可能会导致改善食品安全和延长保质期.
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