人类血病的诊断:系统审查和元分析
Mariana Lourenço Freire1, Tália Santana Machado de Assis1,2, Sarah Nascimento Silva1
1Grupo de Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Brasil.
PLoS neglected tropical diseases
|March 7, 2024
概括
准确诊断牛病是具有挑战性的. 孟加拉,IgG/IgM ELISA和PCR测试的准确性很高,但证据的确定性很低,需要进一步的研究和标准化,以更好地检测血病.
科学领域:
- 医学微生物学 医学微生物学
- 传染性疾病 传染性疾病
- 诊断准确性研究 诊断准确性研究
背景情况:
- 兽是一种广泛传播的动物传染病,由于非特异性症状和报告不足,诊断具有挑战性.
- 准确和及时的诊断对于患者管理和公共卫生控制brucellosis至关重要.
- 目前还没有对人类血病的诊断测试进行全面的比较审查.
研究的目的:
- 系统地审查和评估可用测试的诊断准确性,以确认人类血病.
- 将各种诊断方法的性能与已建立的参考标准进行比较.
主要方法:
- 对2023年1月之前发表的原始研究进行系统审查.
- 包含疑似牛病的症状患者的研究,报告敏感性和/或特异性.
- 排除采用小样本 (<10) 的研究,复杂的乳头病例或检测信息不足的研究.
- 使用"培养"和"培养和标准管凝结试验 (SAT) "作为参考标准.
- 使用 QUADAS-2 的偏见评估和使用 GRADE 工具的证据确定性.
主要成果:
- 包括38项关于人类血清病诊断测试表现的研究.
- 在研究中观察到显著的变化和有限的证据.
- 文化和SAT相结合是比单独的文化更合适的参考标准.
- 孟加拉测试,IgG/IgM ELISA和PCR证明了高的诊断性能.
- 这些高性能测试的证据确定性被评为非常低.
结论:
- 孟加拉测试,IgG/IgM ELISA和PCR显示出布鲁塞洛症诊断的前景.
- 实施需要考虑当地环境和资源.
- 标准化,改进报告和测试开发对于提高血病诊断准确性和可访问性至关重要.
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