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体蛋白质改变发育中的精子的长非编码RNA和DNA,从而诱导父性致死性

Rupinder Kaur^1,2,3, Angelina McGarry^1,2, J Dylan Shropshire^3,4

¹Pennsylvania State University, Departments of Biology and Entomology, University Park, PA 16802, USA.

Science (New York, N.Y.)

|March 7, 2024

概括

来自Wolbachia的体蛋白CifA和CifB改变了Drosophila精子中的长非编码RNA和DNA,通过细胞质不相容性 (CI) 导致胚胎死亡. 这揭示了影响昆虫进化和媒介控制的关键共生机制.

科学领域:

分子生物学
遗传学
进化生物学

背景情况:

传导蛋白与真核巨分子的相互作用尚不清楚.
沃尔巴基亚内共生体通过细胞质不相容性 (CI) 等机制影响宿主繁殖.

研究的目的:

调查沃尔巴基亚传导蛋白CifA和CifB影响多虫精子发育的分子机制,并确定CI.
阐明CifA和CifB在精子生成过程中改变长非编码RNA (lncRNA) 和DNA中的作用.

主要方法:

分析CifA和CifB蛋白作为核糖酶 (RNase) 和脱氧核糖酶 (DNase) 的功能.
研究CifA和CifB对Drosophila精子中的lncRNA水平和DNA完整性的影响.
使用 lncRNA 淘汰和突变来将分子变化与 CI 联系起来.

主要成果:

CifA (RNase) 消耗了精子细胞中一个关键的 lncRNA,这对基因素转化为质氨酸至关重要.
在晚期精子生成过程中,CifA和CifB (DNases) 诱导DNA损伤.
lncRNA的消耗会加剧CI, 分子变化与胚胎DNA损伤相关.

结论:

体蛋白CifA和CifB在游戏生成过程中直接与真核巨分子 (lncRNA和DNA) 相互作用.
这些相互作用对于建立细胞质不相容性至关重要,这是昆虫进化的基本共生.
了解这些机制对昆虫载体控制策略有影响.

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