素基因相关的基因调节的巨细胞表型变异调节早期骨愈合中的血管生成
Qingci Kong1, Siyong Gao1, Pugeng Li1
1Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, Guangdong, China; Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510080, Guangdong, China.
International immunopharmacology
|March 7, 2024
概括
素基因相关 (CGRP) 促进M2巨细胞的两极分化,并在骨愈合过程中增强血管生成,可能是通过上调IL-6来实现. 这种在调节巨细胞功能,有效修复骨方面发挥着关键作用.
科学领域:
- 生物医学工程 生物医学工程
- 再生医学是一种再生医学.
- 免疫学 免疫学 免疫学
背景情况:
- 骨愈合是一个复杂的过程,涉及炎症,增殖和重塑.
- 大细胞两极分化 (M1/M2表型) 极大地影响骨愈合和血管生成.
- 素基因相关 (CGRP) 涉及各种生理过程,包括神经功能和骨代谢.
研究的目的:
- 研究CGRP在早期骨愈合期间调节巨细胞表型中的作用.
- 在骨缺陷模型中确定CGRP对巨细胞调节血管生成的影响.
- 阐明CGRP对巨细胞作用的潜在分子机制.
主要方法:
- 建立鼠下缺陷模型与下膜神经截切 (IANT) 或CGRP受体对抗剂.
- 在体内和体外评估骨质生成,血管生成和巨分化.
- 对CGRP对巨受条件介质和人静脉内皮细胞 (HUVEC) 血管生成的影响的分析.
主要成果:
- CGRP阻塞模仿了IANT效应,抑制骨质生成和血管生成,延长M1巨细胞透,并抑制M1到M2的过渡.
- 在体外,CGRP通过YES相关蛋白1激活促进了M2巨细胞的极化,并通过YES相关蛋白1激活在M1巨细胞上调调节了IL-6的表达.
- 来自CGRP治疗的巨细胞的条件介质比直接CGRP治疗更多地增强了HUVEC血管生成.
结论:
- CGRP影响M1到M2巨细胞的表型变化,可能是通过M1巨细胞的IL-6上调.
- 通过调节巨细胞功能,CGRP在早期骨愈合中显示出亲血管性潜力.
- 这些发现凸显了CGRP作为增强骨修复的潜在治疗点.
相关概念视频
Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Hormones and Bone Tissue
2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K


