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相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Overview of Secretory Vesicles

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Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
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Glycocalyx and its Functions01:14

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The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
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Mechanical Protein Function01:58

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Regulation of the Unfolded Protein Response01:31

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Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
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Bacterial Signaling01:30

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Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
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相关实验视频

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Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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EvfG是一种位于ExPEC的VI型分泌系统中的多功能蛋白质.

Wenjia Lu1, Hao Lu2, Xinyu Huo2

  • 1National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; Hubei Hongshan Laboratory, Wuhan, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China.

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|March 7, 2024
PubMed
概括

一个新发现的基因evfG调节了致病性大肠杆菌的第六类分泌系统 (T6SS). 该基因调节细菌的竞争,应激反应和毒性,为药物开发提供了新的途径.

关键词:
在C-di-GMP中目标药物是药物目标.移动性 移动性 移动性氧化应激是一种氧化应激.在T6SSSS中使用.evfG 的意思是

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相关实验视频

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科学领域:

  • 微生物学 微生物学
  • 细菌病原体的产生
  • 分子生物学分子生物学

背景情况:

  • 六型分泌系统 (T6SS) 是一种关键的细菌纳米武器,参与了细菌之间的竞争和毒性.
  • 在肠外致病性大肠杆菌 (ExPEC) 中对T6SS的研究是有限的,关键的调节机制未被充分探索.

研究的目的:

  • 在ExPEC中识别和描述调节T6SS功能的新型基因.
  • 阐明未表征基因evfG在ExPEC RS218 T6SS活动和细菌特征中的作用.

主要方法:

  • 在T6SS集群中的基因鉴定.
  • 对evfG在T6SS表达和活动中的作用进行实验分析.
  • 研究evfG与c-di-GMP和soda的相互作用.
  • 转录组分析和额外的测试以评估evfG控制的特征.

主要成果:

  • 确定并确认evfG基因参与了ExPEC RS218.18中的T6SS表达.
  • EvfG通过竞争性结合c-di-GMP来调节T6SS活动,减少其抑制作用.
  • 为了减轻氧化应激,EvfG招募了SODA.
  • EvfG调节多种细菌特征,包括附着性,入侵性,运动性,耐药性和致病性.

结论:

  • 在ExPEC中,EvfG充当T6SS和相关细菌活动的多功能调节器.
  • 了解evfG的作用有助于推进T6SS研究,并为新型疫苗和对抗ExPEC感染的治疗策略提供机会.