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安纳米德对脊柱感知传输在控制和炎症条件下的双重作用
Monica Pontearso1, Jakub Slepicka1, Anirban Bhattacharyya1
1Laboratory of Pain Research, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
概括
胺 (AEA) 对疼痛信号产生双重影响,在炎症下抑制它,但在正常情况下具有混合作用. 调节AEA产生可能比直接准其受体提供更好的疼痛缓解.
科学领域:
- 神经科学是一个神经科学.
- 疼痛研究 疼痛研究
- 内分泌大麻素系统 内分泌大麻素系统
背景情况:
- 安南胺 (AEA) 通过大麻素 (CB1) 和TRPV1受体调节脊柱的 nociception.
- 它在正常和炎症条件下的突触传播中的作用需要进一步阐明.
研究的目的:
- 研究安纳米德 (AEA) 对感觉突触传输的调节作用.
- 检查AEA对脊髓切片中微型刺激后突触电流 (mEPSC) 的影响,控制和炎症状态下.
主要方法:
- 从脊髓神经元记录的MEPSCs.
- 应用AEA (1-30μM) 单独和与CB1受体对抗剂 (PF514273),TRPV1对抗剂 (SB366791) 和FAAH抑制剂 (URB597) 的组合.
- 在原始和炎症条件下的效果比较.
主要成果:
- 在原始条件下,AEA表现出混合作用,只有当CB1和FAAH被抑制时才观察到激发.
- 炎症将AEA的影响转移到抑制方面,较高比例的神经元响应.
- 更多的神经元在炎症后表现出AEA和抑制剂的mEPSC频率增加.
结论:
- 在第一个可感应突触上,AEA表现出双重刺激和抑制作用,并复杂化了针对内分泌系统的止痛药的开发.
- 与直接应用AEA前体相比,调节内源性AEA生产可能是止痛治疗中更有前途的策略.
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