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Updated: Jul 25, 2026

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抗生素对Vibrio fischeri的长期毒性评估:测试方法的优化和混合物毒性预测

Shuhan Ge¹, Wanqing Tian¹, Ziyi Lou¹

¹Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science & Engineering, Shandong University, Qingdao, Shandong 266237, China.

Journal of hazardous materials

|March 7, 2024

概括

一种新方法通过标准化与Vibrio fischeri细菌接触时间来准确评估长期抗生素毒性. 这揭示了对抗生素风险的严重低估,特别是混合物,需要更严格的排放法规.

科学领域:

环境科学环境科学
微生物学微生物学
毒理学毒理学毒理学

背景情况:

目前针对急性抗生素毒性的发光细菌测试是不可靠的,因为接触时间短且可变.
现有的长期毒性评估缺乏标准化的接触时间,导致无与伦比的结果.

研究的目的:

建立使用Vibrio fischeri.使用抗生素的统一的长期毒性评估方法.
量化抗生素及其混合物在水生环境中的低估毒性.
研究抗生素相互作用及其协同作用.

主要方法:

开发了一种标准化的长期毒性测试,具有统一的接触时间 (生物发光增长期),用于抗生素和Vibrio fischeri.
采用相当度加法 (ECA) 模型,根据物理机制预测抗生素混合物的毒性.
分析了抗生素混合物的协同抑制效应,包括宏类和类.

主要成果:

新方法揭示了抗生素半最大抑制度 (IC50) 比急性测试低2-3个数量级,突出显示了低估的毒性.
该ECA模型准确预测了混合物的毒性 (R2 = 0.94).
在细菌生长过程中作用的抗生素,如宏类和类,表现出强烈的协同抑制;一些,如宏类,表现出随着度积累而增加的协同抑制.

结论:

已确定的方法提供了对长期抗生素毒性的可靠评估.
抗生素混合物具有显著的协同作用风险,需要谨慎的环境管理.
具有严重的长期毒性和强大的协同作用的抗生素的排放需要严格的监管.

关键词:

生物发光是一种生物发光.度添加模型的模型.生态风险生态风险霍尔梅西斯 (Hormesis) 是一个协同作用的抑制作用.

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