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低化度和应用时间对ex vivo多微生物生物膜模型中的细菌的影响

Hazuki Maezono¹, Kittipit Klanliang², Tsuyoshi Shimaoka¹

¹Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.

Journal of endodontics

|March 7, 2024

概括

低化 (NaOCl) 有效地杀死根运河生物膜中的细菌. 较高度和较长的NaOCl暴露时间更有效,成熟的生物膜表现出更高的耐药性.

科学领域:

牙周内科医院牙周内科医院牙周内科医院
微生物学微生物学
生物材料是一种生物材料.

背景情况:

生物膜的去除在内牙治疗中至关重要.
调查低酸盐 (NaOCl) 对根管细菌的疗效至关重要.

研究的目的:

评估不同度和应用时间对 ex vivo 多微生物生物膜内的细菌活力的影响.
评估生物膜成熟对NaOCl有效性的影响.

主要方法:

活体多微生物生物膜被培养了1,2或3周.
生物膜被暴露在NaOCl (0.1%-2%) 中1或3分钟.
使用共聚焦激光扫描显微镜和扫描电子显微镜评估了细菌的活力.

主要成果:

与对照组相比,0.5%-2%的NaOCl度显著降低了细菌活力 (P < .05).
长时间暴露 (3分钟) 导致细菌活力低于短时间暴露 (1分钟).
随着度更高的NaOCl和更长的应用时间,细菌杀死率增加.

结论:

较高的NaOCl度和较长的应用时间增强了根运河生物膜中的细菌杀死.
成熟的生物膜与年轻的生物膜相比,对NaOCl处理具有更大的抵抗力.
NaOCl的有效性是度和时间依赖的,生物膜成熟度会影响敏感性.

关键词:

生物膜是一种生物膜.牙科消毒剂牙科消毒剂牙斑块是指牙上的斑块.根管灌剂根管灌剂盐酸盐酸盐酸盐的过化物

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