食批次发酵的Mucor circinelloides透露显著改善生物质和脂质积累通过性能评估,化学分析和表达形状
Hassan Mohamed1, Tahira Naz2, Qing Liu2
1Colin Ratledge Center of Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, China; Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt.
Bioresource technology
|March 7, 2024
概括
对Mucor circinelloides WJ11进行了优化,提高了脂质和生物质产量. 战略S1实现了最高的生物质和脂质含量,证明了油性真菌有效的葡萄糖联合养.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 脂质的生产 脂质的生产
背景情况:
- 粘真菌 (Mucor circinelloides WJ11) 是一种具有可能产生脂质的真菌.
- 优化发酵条件对于最大限度地提高生物质和脂质产量至关重要.
研究的目的:
- 为了提高Mucor circinelloides WJ11的脂质和生物质产量.
- 评估四种不同的料批发发酵策略,其时间和葡萄糖度各不相同.
- 为了确定最佳的非生物因素,以有效地生产脂质.
主要方法:
- 实施四种料批发发酵策略 (S1-S4),使用不同的葡萄糖度和发酵时间.
- 使用定量逆转录聚合酶链反应 (RT-qPCR) 分析与脂质积累相关的基因转录.
- 使用富里埃变换红外光谱法 (FTIR) 进行生物化学分析.
主要成果:
- 120-144小时后,S1策略的结果是生物质 (22 g/L),脂质含量 (53%) 和脂肪酸含量 (28%) 的最高值.
- 策略S3在48小时内产生了最高的玛-烯酸标位 (0.75g/L).
- 观察到葡萄糖-6-酸盐脱酶,6-酸酸盐脱酶和ATP-酸酶基因的表达增加.
- 最佳条件包括pH6.5,25-26°C,15%的注射剂,500rpm,20-30%的溶氧和120小时的发酵.
结论:
- 料批发发酵,特别是S1策略,显著提高了Mucor circinelloides WJ11中的脂质和生物质产量.
- 葡萄糖联合养是一种有价值的方法,用于开发微生物脂质生产的有效发酵策略.
- 了解基因表达和生化特征有助于优化油性真菌的发酵过程.
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