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Updated: Jul 1, 2025

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Cas9辅助生物制一种基因工程的人类共生细菌和遗传元素

Naoki Hayashi^1,2, Yong Lai^3,4,5, Jay Fuerte-Stone⁶

¹Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Nature communications

|March 7, 2024

概括

用于肠道微生物组研究的工程细菌需要强大的生物控制. 一个新的Cas9辅助系统使用蒂米丁辅和CRISPR技术,通过防止遗传逃逸和传播来确保安全.

科学领域:

合成生物学合成生物学
微生物组研究的研究.
基因工程是一种基因工程.

背景情况:

工程细菌有可能用于肠道微生物组诊断和治疗.
强大的生物封闭对于转基因生物体的安全部署至关重要.

研究的目的:

开发和验证人类肠道微生物组中工程细菌的新型生物封闭系统.

主要方法:

设计了一种Cas9辅助的辅助性生物控制系统,集成了提米丁辅助性,工程性肋骨调节器 (ER) 和CRISPR设备 (CD).
该系统在人类肠道共生细菌 Bacteroides thetaiotaomicron 中进行了测试.
验证包括体外和体内研究.

主要成果:

该系统实现了对基因表达的调节控制.
它成功地阻止了工程细菌获得thyA基因,这对于胺素原性至关重要.
转基因传播被有效地阻止,在特定条件下维持了细菌的活力.

结论:

开发的Cas9辅助辅食生物控制系统为工程细菌提供了强大的安全性.
这一战略使得基因工程微生物在生物医学中的安全应用成为可能,特别是在肠道微生物群中.

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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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