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基因表达阵列分析预测了胰腺管道腺癌在围神经侵入期间的原基因表达

Shu Zhao¹, Zhen Xue², Jing Yao Wang³

¹Department of Medical Oncology, The Second Medical Center and National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, China.

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology

|March 7, 2024

概括

这项研究确定了关键基因和参与胰腺癌近神经入侵的nestin/血管内皮生长因子途径. 这些发现为胰腺管道腺癌提供了新的预后标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

胰腺管道腺癌 (PDAC) 呈现出高频率的围神经侵入.
了解驱动PDAC围神经侵入的分子机制对于改善患者的治疗结果至关重要.

研究的目的:

为了识别带有或没有围神经侵袭的PDAC组织之间的差异表达基因.
发现关键的基因和与PDAC围神经侵入和预后相关的途径.

主要方法:

对GSE102238基因表达特征的分析.
基因功能和通路丰富分析.
构建蛋白质-蛋白质相互作用网络以识别枢纽基因.
预后因素的单变量和多变量考克斯回归分析.
使用定量RT-PCR和整体生存分析验证枢纽基因.

主要成果:

确定了242个不同表达的基因 (68个上调,174个下调).
发现了九个核心基因和与围神经侵入相关的nestin (NES) /血管内皮生长因子 (VEGF) 信号通路.
差异化成为一个独立的预后因素 (P < .05).
经验证的三个上调基因 (JUP,CALM1,NES) 和六个下调基因 (EPHA2,ARF1,ORM2,TERT,IL18,CXCL3) 的总体存活率明显降低 (P < .05).

结论:

九个核心基因和NES/VEGF通路与PDAC围神经侵入有关.
这些基因和差异化状态作为PDAC周围神经侵入的潜在预后指标.

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