相关实验视频
Updated: Jul 1, 2025
05:39
Spontaneous Murine Model of Anaplastic Thyroid Cancer
Published on: February 3, 2023
1.6K
通过维持氧化还原平衡,ENO2促进甲状腺癌的抗性
Yu Zhang1,2, Xiaoyu Ji3, Yu Wang1,2
1Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.
Gland surgery
|March 8, 2024
概括
无塑性甲状腺癌细胞通过乙醇酶2 (ENO2) 和氧化还原平衡抵抗阿诺基斯,阻碍转移. 抑制ENO2在体内减少肺部殖民,提供治疗潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症转移 癌症转移
背景情况:
- 阿诺基斯,或依赖结的细胞死亡,是癌症转移的关键障碍.
- 无塑性甲状腺癌 (ATC) 是高度侵略性的,具有显著的转移潜力和死亡率.
- 了解ATC中阿诺基斯抵抗机制对于开发有效的治疗策略至关重要.
研究的目的:
- 为了研究乙醇酶2 (ENO2) 在抗甲状腺癌中抗甲状腺耐药性的作用.
- 为了阐明ENO2介导的基抗性背后的分子机制.
- 评估针对ATC转移中的ENO2的治疗潜力.
主要方法:
- 用RNA测序来识别附着与分离的ATC细胞中的差异表达基因.
- 功能增益和丧失研究,以评估ENO2对亡和球形形成的影响.
- 测量活性氧物种 (ROS),谷氨 (GSH) 和NADPH,以评估还原氧平衡.
- 双露西法酶记者测试验证了STAT1对ENO2.2的转录调节.
- 在裸体小鼠体内研究,以评估ENO2抑制对肺转移的作用.
主要成果:
- 在脱离的ATC细胞中,ENO2显著上调,与激活的糖解相关.
- 通过提供GSH和NADPH,ENO2的过度表达维持了氧化还原平衡,从而赋予了阿诺基斯的抗性.
- 信号转换器和转录1的激活器 (STAT1) 在耐阿诺基斯细胞中积极调节ENO2表达.
- 在ATC细胞中抑制ENO2在体内显著降低了肺部殖民.
结论:
- 稳定的ENO2表达对于形甲状腺癌中阿诺基斯耐药性至关重要.
- 通过ENO2维持氧化还原平衡是促进ATC转移的关键机制.
- 向ENO2为控制ATC进展和转移提供了一个有希望的治疗途径.
相关概念视频
01:46Electron Transport Chain: Complex I and II
13.3K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
13.3K
02:53Adaptive Mechanisms in Cancer Cells
5.8K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.8K
01:30Apoptosis
11.4K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
11.4K
02:49Cancer Therapies
7.7K
Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
7.7K
01:18Functions of Thyroid Hormones
2.7K
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
2.7K
01:30Overview of Cell Death
7.3K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.3K