相关实验视频

Updated: Jul 1, 2025

Microscopy of Fission Yeast Sexual Lifecycle

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

最初的营养条件决定了裂变酵母酵母中静止细胞的恢复速度

Qi Liu^1,2, Nan Sheng¹, Zhiwen Zhang³

¹Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.

|March 8, 2024

概括

静止细胞是静止的细胞.

科学领域:

微生物学和细胞生物学
定量生物学定量生物学
系统生物学系统生物学

背景情况:

微生物细胞由于环境压力,大部分时间都处于静止状态.
静止细胞具有恢复能力,包括生存能力和恢复速度.
以前的研究集中在衰老期间的生存能力上,忽视了恢复动态.

研究的目的:

量化分析静止裂变酵母的恢复活动和生存能力.
调查饥饿时间和营养条件对恢复动态的影响.
了解静止细胞再生的进化动态.

主要方法:

在单细胞水平上对生存能力和恢复活动的系统量化.
对长寿静止裂变酵母进行的实验.
转录组分析分析静止期间的基因表达变化.

主要成果:

单细胞恢复时间与生存率显著下降之前的饥饿时间线性增加.
恢复速度在不同的营养条件下是稳定的,并根据初始条件预先确定.
转录组数据揭示了静态进化的共同轨迹,速度各不相同.

结论:

细胞静止存在于一个连续的深度谱.
环境条件显著影响静止细胞的生理学.
恢复速度是细胞静止的一个关键,量化可预测的方面.

关键词:

动力学动力学动力学裂变酵母酵母是一种裂变酵母.长时间的静止.的饥饿是因为的饥饿恢复活动恢复活动.一个单细胞研究.时间延迟成像技术的时间延迟成像.

更多相关视频

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing

Published on: July 17, 2019

相关概念视频

Cells Coordinate Growth and Proliferation

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...