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牙的器官型模型用于研究涉及牙周炎的细菌和病毒病原体

Anna Golda¹, Anna Gasiorek¹, Ewelina Dobosz¹

¹Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University, Krakow, Poland.

Journal of oral microbiology

|March 8, 2024

概括

一个新的3D有机型牙 (OTG) 模型有效地模仿人类牙,以研究牙周炎病原体,如* Porphyromonas gingivalis*和HSV-1,并测试抗菌药物的有效性.

科学领域:

口腔微生物学口腔微生物学
组织工程是组织工程.
牙周病学牙周病学

背景情况:

三维 (3D) 组织模型为2D培养和动物模型提供了更现实的替代方案.
开发先进的模型对于了解牙周炎等复杂的口腔疾病至关重要.

研究的目的:

开发一个有机型的3D牙 (OTG) 模型.
用OTG模型来研究参与牙周炎的细菌和病毒病原体.

主要方法:

使用牙纤维细胞 (GFs) 和永生牙角质细胞 (TIGKs) 构建了OTG模型.
研究的感染是由 * Porphyromonas gingivalis * 和简单疹病毒 1 (HSV-1).
采用免疫组织化学,共聚焦显微镜,qPCR,定位和CFU计数来分析病原体的存在,免疫反应和药物疗效.

主要成果:

OTG模型准确地复制了人类的牙形态.
这两种病原体都透到组织中并持续存在; *P. gingivalis* 形成生物膜.
感染诱导了炎症媒介,而抗微生物药物有效清除了病原体.

结论:

OTG模型是一种简单,方便的工具,用于研究牙组织中的病原体相互作用 (透,持久性,生物膜形成).
该模型适用于评估抗微生物治疗对口腔病原体的有效性.

关键词:

机体类型的器官类型.葡萄牙牙牙病 Porphyromonas gingivalis 这种虫害的生物膜是一种生物膜.牙模型牙模型牙组织牙组织简单疹病毒-1-1 感染的感染感染.持续性持久性

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